Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606230436_fdp2 ATTATTGAACTATTCATTCATTTAGCCTAAAG(+ATATAT)GATCCAAGGTGTTAGAGTTTCTTTCCCTATG NGG 10 8 17 8 24 0 100 5 10
    240606230434_smm7 TGGGGCATAGGGAAAGAAACTCTAACACCTTG(+ATATAT)GATCCTTTAGGCTAAATGAATGAATAGTTCA NGG 10 8 17 8 24 0 100 5 10
    240606230432_1h1p GGATGGACCATCTAGTGACTGCCATATGCAGG(+ATATAT)GATCCATCCCATAATTAGCTTCCAAATGCTG NGG 10 8 17 8 24 0 100 5 10
    240606230428_87mf GGTGTCAGCATTTGGAAGCTAATTATGGGATG(+ATATAT)GATCCCTGCATATGGCAGTCACTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240606230426_m6t8 AGACCAGTGTGGAAAGGACCGTGGGGTCCTTG(+ATATAT)GATCCTCACTTCCTGATATCAAGCTACCACT NGG 10 8 17 8 24 0 100 5 10
    240606230424_dbda AGAACAGTGGTAGCTTGATATCAGGAAGTGAG(+ATATAT)GATCCAAGGACCCCACGGTCCTTTCCACACT NGG 10 8 17 8 24 0 100 5 10
    240606230422_4vsp CCACTTTATCATAGAAATACCCCACTCAGCTG(+ATATAT)GTACCAATTTGCCTTAGTCATGGTTTCTATT NGG 10 8 17 8 24 0 100 5 10
    240606230421_i9sx CCAGGAATAGAAACCATGACTAAGGCAAATTG(+ATATAT)GTACCAGCTGAGTGGGGTATTTCTATGATAA NGG 10 8 17 8 24 0 100 5 10
    240606230419_gqkr CTCACCTCCCAGGTCTCTGGTACATTCTGGAG(+ATATAT)GATCCCTGCACCTCTTTCTTCCCAAGGTTGT NGG 10 8 17 8 24 0 100 5 10
    240606230417_g3zu AACAAACAACCTTGGGAAGAAAGAGGTGCAGG(+ATATAT)GATCCTCCAGAATGTACCAGAGACCTGGGAG NGG 10 8 17 8 24 0 100 5 10