Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606230355_468y CTTAATGCCCAGTGTGTTTGGCAATATTTCAG(+ATATAT)GATCCGAAAATTATTCAAATTTTGTTTGTTA NGG 10 8 17 8 24 0 100 5 10
    240606230354_43yh TTTAATAACAAACAAAATTTGAATAATTTTCG(+ATATAT)GATCCTGAAATATTGCCAAACACACTGGGCA NGG 10 8 17 8 24 0 100 5 10
    240606230352_ensi ACATGTCTACACTTCTTAGATCCTTAGTTATG(+ATATAT)GATCCAGTTTTCACATTTCCAGGCCTAACCT NGG 10 8 17 8 24 0 100 5 10
    240606230351_f953 AGCAAAGGTTAGGCCTGGAAATGTGAAAACTG(+ATATAT)GATCCATAACTAAGGATCTAAGAAGTGTAGA NGG 10 8 17 8 24 0 100 5 10
    240606230348_pspe CTACCCTTGACACAGGAAGTATATCCTAAAGG(+ATATAT)GATCCGTCGTAGGACACAGCCCTACCCTGGT NGG 10 8 17 8 24 0 100 5 10
    240606230346_rf05 TGTTCACCAGGGTAGGGCTGTGTCCTACGACG(+ATATAT)GATCCCTTTAGGATATACTTCCTGTGTCAAG NGG 10 8 17 8 24 0 100 5 10
    240606230345_xxzr CCGGAGGAAACATGAGCGGGATCGTCTGAGCG(+ATATAT)GATCCCTTTCCCTGGCTGTGTGCGCGTGCGT NGG 10 8 17 8 24 0 100 5 10
    240606230343_md8r ACTCCACGCACGCGCACACAGCCAGGGAAAGG(+ATATAT)GATCCGCTCAGACGATCCCGCTCATGTTTCC NGG 10 8 17 8 24 0 100 5 10
    240606230341_76e1 AGGAACTCAGGCTGACCTCAGACTCCATGCAG(+ATATAT)GATCCTCCTGTCAGAGCCATGATGACATAAA NGG 10 8 17 8 24 0 100 5 10
    240606230339_jjt8 GTTGCTTTATGTCATCATGGCTCTGACAGGAG(+ATATAT)GATCCTGCATGGAGTCTGAGGTCAGCCTGAG NGG 10 8 17 8 24 0 100 5 10