Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606225837_tucv GTCCCTGGTTCTGCTCAAACAGTTACTTCAAG(+ATATAT)GTACCCTTAAAAGGGCTCCATTAAACAAGAA NGG 10 8 17 8 24 0 100 5 10
    240606225835_pgku GTACATTCTTGTTTAATGGAGCCCTTTTAAGG(+ATATAT)GTACCTTGAAGTAACTGTTTGAGCAGAACCA NGG 10 8 17 8 24 0 100 5 10
    240606225833_hsuv TAGGCAGGAACTGGGGACTAGAGAAGCATCTG(+ATATAT)GTACCAACACCACAGACTTCTTCAAGCTAAT NGG 10 8 17 8 24 0 100 5 10
    240606225832_l6gg TTTCAATTAGCTTGAAGAAGTCTGTGGTGTTG(+ATATAT)GTACCAGATGCTTCTCTAGTCCCCAGTTCCT NGG 10 8 17 8 24 0 100 5 10
    240606225830_y20j CTTGACCGATTAGCTCGCATTTTATCTGGCTG(+ATATAT)GTACCCTTTTCCCTCATAATGAGCTGCTTCT NGG 10 8 17 8 24 0 100 5 10
    240606225828_crz7 GAGGAAGAAGCAGCTCATTATGAGGGAAAAGG(+ATATAT)GTACCAGCCAGATAAAATGCGAGCTAATCGG NGG 10 8 17 8 24 0 100 5 10
    240606225826_psd4 AAAAGGATATACCTTCTTCTCAGCACCTCATG(+ATATAT)GTACCTTCTCCAAAATTGACCACATAATAGG NGG 10 8 17 8 24 0 100 5 10
    240606225824_gtku TGTGACCTATTATGTGGTCAATTTTGGAGAAG(+ATATAT)GTACCATGAGGTGCTGAGAAGAAGGTATATC NGG 10 8 17 8 24 0 100 5 10
    240606225822_m25w ACGAAATGGACAAATTTCTGGACAGATACCAG(+ATATAT)GTACCAAAGTTGAATCAGGATCAAGTTGACC NGG 10 8 17 8 24 0 100 5 10
    240606225820_990i TAGAAGGTCAACTTGATCCTGATTCAACTTTG(+ATATAT)GTACCTGGTATCTGTCCAGAAATTTGTCCAT NGG 10 8 17 8 24 0 100 5 10