Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606225755_gl61 TCCTCATAAAATTGGACATAGTACTACCGGAG(+ATATAT)GATCCAGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240606225754_31ur TTCTGGATATATGCCCAGGAGAGGTATTGCTG(+ATATAT)GATCCTCCGGTAGTACTATGTCCAATTTTAT NGG 10 8 17 8 24 0 100 5 10
    240606225751_2v3y GGATGGACCATCTAGTGATTGCCATATCCAGG(+ATATAT)GATCCATCCCATAATCAGCTTCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240606225749_r84z GGTGTCAGCGTTTGGAAGCTGATTATGGGATG(+ATATAT)GATCCCTGGATATGGCAATCACTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240606225746_0qap TGAGCTCTTAGCCCTAGGTTTTAAGAGCCTCG(+ATATAT)GATCCCAACTATGTTGAATTTCCTAAACTAC NGG 10 8 17 8 24 0 100 5 10
    240606225745_3e6s ACATCGTAGTTTAGGAAATTCAACATAGTTGG(+ATATAT)GATCCGAGGCTCTTAAAACCTAGGGCTAAGA NGG 10 8 17 8 24 0 100 5 10
    240606225742_lr6i TCCTCAGAAAATTGGACATAGTACTACCGGAG(+ATATAT)GATCCAGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240606225740_8i4m TTCTGGATATATGCCCAGGAGAGGTATTGCTG(+ATATAT)GATCCTCCGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240606225737_7zcz GGGTGGACCATGTAGAGACTGCCTTATCCAGG(+ATATAT)GATCCACCCCATAATCAGCATCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240606225736_wvq5 GGTGTCAGCGTTTGGATGCTGATTATGGGGTG(+ATATAT)GATCCCTGGATAAGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10