Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606225656_utp5 ATTTCTTCAAAGAGAGACAAGGTTCATGCCTG(+ATATAT)GTACCATTTTTTTTCTAATGAAATTAACTTT NGG 10 8 17 8 24 0 100 5 10
    240606225654_ctxo CTAAAAAAGTTAATTTCATTAGAAAAAAAATG(+ATATAT)GTACCAGGCATGAACCTTGTCTCTCTTTGAA NGG 10 8 17 8 24 0 100 5 10
    240606225652_ficp GTGGTGCACTCCAACAGCATTTTAAGTACTTG(+ATATAT)GATCCCACGTTGATCTTATAAACACAAGCAA NGG 10 8 17 8 24 0 100 5 10
    240606225650_6wlb TTTTATTGCTTGTGTTTATAAGATCAACGTGG(+ATATAT)GATCCAAGTACTTAAAATGCTGTTGGAGTGC NGG 10 8 17 8 24 0 100 5 10
    240606225648_68z0 TCTCTGTCTGACTTCCTATGCATGGGGGCTGG(+ATATAT)GATCCATTTCTAAGGAGAATGGATAAGGAAG NGG 10 8 17 8 24 0 100 5 10
    240606225647_asxb GCCTACTTCCTTATCCATTCTCCTTAGAAATG(+ATATAT)GATCCCAGCCCCCATGCATAGGAAGTCAGAC NGG 10 8 17 8 24 0 100 5 10
    240606225645_5x76 GTGGTGCACTCCAACAGCATTTTAAGTACTTG(+ATATAT)GATCCCACGTTGATCTTATAAACACAAGCAA NGG 10 8 17 8 24 0 100 5 10
    240606225643_079v TTTTATTGCTTGTGTTTATAAGATCAACGTGG(+ATATAT)GATCCAAGTACTTAAAATGCTGTTGGAGTGC NGG 10 8 17 8 24 0 100 5 10
    240606225641_hovq TCTCTGTCTGACTTCCTATGCATGGGGGCTGG(+ATATAT)GATCCATTTCTAAGGAGAATGGATAAGGAAG NGG 10 8 17 8 24 0 100 5 10
    240606225640_bwnp GCCTACTTCCTTATCCATTCTCCTTAGAAATG(+ATATAT)GATCCCAGCCCCCATGCATAGGAAGTCAGAC NGG 10 8 17 8 24 0 100 5 10