Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606225602_mwa0 GGGATTCTACCGTGCACTGCTGCATCCAGTAG(+ATATAT)GATCCTAGCTAAAGGCTTTCTCTACTATGAT NGG 10 8 17 8 24 0 100 5 10
    240606225601_be29 ACAACATCATAGTAGAGAAAGCCTTTAGCTAG(+ATATAT)GATCCTACTGGATGCAGCAGTGCACGGTAGA NGG 10 8 17 8 24 0 100 5 10
    240606225559_fla2 AATCTTTTCAGAGAATTCTAAAAAGACTATAG(+ATATAT)GATCCTATAATAGATCCAATGATCTATAGTG NGG 10 8 17 8 24 0 100 5 10
    240606225558_82h0 TATGACACTATAGATCATTGGATCTATTATAG(+ATATAT)GATCCTATAGTCTTTTTAGAATTCTCTGAAA NGG 10 8 17 8 24 0 100 5 10
    240606225556_szcv GCTCACAACTGTTCGTAACTCAAGTTTCAGAG(+ATATAT)GATCCAGTGCCCTCTTTGGACTCCCACAGGC NGG 10 8 17 8 24 0 100 5 10
    240606225554_hxlp CTGGTGCCTGTGGGAGTCCAAAGAGGGCACTG(+ATATAT)GATCCTCTGAAACTTGAGTTACGAACAGTTG NGG 10 8 17 8 24 0 100 5 10
    240606225553_g0rn TTGAGTTGAACTAGTGTTGAGGCTAGGCTTAG(+ATATAT)GTACCATAGAACTTTGGTTTTTCAAGACAGG NGG 10 8 17 8 24 0 100 5 10
    240606225552_26hu GAAACCCTGTCTTGAAAAACCAAAGTTCTATG(+ATATAT)GTACCTAAGCCTAGCCTCAACACTAGTTCAA NGG 10 8 17 8 24 0 100 5 10
    240606225550_wtwc CTGACTGGCTCTGGCCTATGAACTCATTGCTG(+ATATAT)GATCCATTAACCCTTTCCTAGCTTCAGAGGT NGG 10 8 17 8 24 0 100 5 10
    240606225548_kiit GAATTACCTCTGAAGCTAGGAAAGGGTTAATG(+ATATAT)GATCCAGCAATGAGTTCATAGGCCAGAGCCA NGG 10 8 17 8 24 0 100 5 10