Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606225448_ptp3 TAGGTGACATTCAGAGATGCTGTGTTGGGCTG(+ATATAT)GTACCAGAATGCATTGATGTGTGTGCTTTAC NGG 10 8 17 8 24 0 100 5 10
    240606225447_mdzm CAACGGTAAAGCACACACATCAATGCATTCTG(+ATATAT)GTACCAGCCCAACACAGCATCTCTGAATGTC NGG 10 8 17 8 24 0 100 5 10
    240606225445_wi90 TATGCTACTTGCACTGTGACATGGCATTTGAG(+ATATAT)GATCCCTGACTTACAGACAAAGCTTAAAATG NGG 10 8 17 8 24 0 100 5 10
    240606225443_byu3 TGTGTCATTTTAAGCTTTGTCTGTAAGTCAGG(+ATATAT)GATCCTCAAATGCCATGTCACAGTGCAAGTA NGG 10 8 17 8 24 0 100 5 10
    240606225441_llrt AAAGCTCATCACAAGGTCTACTAAGCATTAAG(+ATATAT)GATCCTCATTATAAGTCCTCTGTACCACTTT NGG 10 8 17 8 24 0 100 5 10
    240606225440_pw45 AAAAAAAAGTGGTACAGAGGACTTATAATGAG(+ATATAT)GATCCTTAATGCTTAGTAGACCTTGTGATGA NGG 10 8 17 8 24 0 100 5 10
    240606225438_zx4a AATACTGACTCTCATTAGATATGATCTTATAG(+ATATAT)GATCCATACCCTCCCTAGGACCAAATATATC NGG 10 8 17 8 24 0 100 5 10
    240606225436_zh83 CATCAGATATATTTGGTCCTAGGGAGGGTATG(+ATATAT)GATCCTATAAGATCATATCTAATGAGAGTCA NGG 10 8 17 8 24 0 100 5 10
    240606225434_3i9e GGCATGGCTCCTCGCCGCAGCTGTCTCCCTTG(+ATATAT)GTACCGACCTCTCTGTGGGCTGGAGAGAGGC NGG 10 8 17 8 24 0 100 5 10
    240606225432_lbyl CTTAGGCCTCTCTCCAGCCCACAGAGAGGTCG(+ATATAT)GTACCAAGGGAGACAGCTGCGGCGAGGAGCC NGG 10 8 17 8 24 0 100 5 10