Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606225430_vgd5 CCTTGTCAAACCAAGTTGTCATGGATGCTTTG(+ATATAT)GTACCCCTCCCCTCAGGGGCTTTGGTATTAC NGG 10 8 17 8 24 0 100 5 10
    240606225428_e7l1 TTCTTGTAATACCAAAGCCCCTGAGGGGAGGG(+ATATAT)GTACCAAAGCATCCATGACAACTTGGTTTGA NGG 10 8 17 8 24 0 100 5 10
    240606225426_pae7 GTGGGGTTCCTGCGCTGTGTGTGCCTACTCAG(+ATATAT)GTACCCCAACATTGCTTTCCCTAGCTCTCTG NGG 10 8 17 8 24 0 100 5 10
    240606225423_3ehp CACAGCAGAGAGCTAGGGAAAGCAATGTTGGG(+ATATAT)GTACCTGAGTAGGCACACACAGCGCAGGAAC NGG 10 8 17 8 24 0 100 5 10
    240606225422_3nrw GCTCAATGCCATCATCACTGTGATTAAGTGAG(+ATATAT)GTACCAGGGTTCATAGCGTCGGAGACTGCTG NGG 10 8 17 8 24 0 100 5 10
    240606225419_gd9d AGCGTCAGCAGTCTCCGACGCTATGAACCCTG(+ATATAT)GTACCTCACTTAATCACAGTGATGATGGCAT NGG 10 8 17 8 24 0 100 5 10
    240606225417_8lmh ACAGGAACCAGTCCCTAAAAGTGCTCTTCTCG(+ATATAT)GTACCTGGCGAGGCAATCATTCCAGCGCTTC NGG 10 8 17 8 24 0 100 5 10
    240606225415_7br3 AAACAGAAGCGCTGGAATGATTGCCTCGCCAG(+ATATAT)GTACCGAGAAGAGCACTTTTAGGGACTGGTT NGG 10 8 17 8 24 0 100 5 10
    240606225413_n7uo AGAATCTAGTAGCCACACAATGGGGGGCTCAG(+ATATAT)GTACCATTATTTACTACTTTCTTTTGACCTA NGG 10 8 17 8 24 0 100 5 10
    240606225412_lmwv AGTCCTAGGTCAAAAGAAAGTAGTAAATAATG(+ATATAT)GTACCTGAGCCCCCCATTGTGTGGCTACTAG NGG 10 8 17 8 24 0 100 5 10