Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606225349_0l6u AGACTAAGCAATAATATTTAGCTCTGGAATGG(+ATATAT)GTACCATCTCCTTTTTCCTCATTAAAGGCTC NGG 10 8 17 8 24 0 100 5 10
    240606225348_85pj TGGTTGAGCCTTTAATGAGGAAAAAGGAGATG(+ATATAT)GTACCCATTCCAGAGCTAAATATTATTGCTT NGG 10 8 17 8 24 0 100 5 10
    240606225345_w0r2 CTTTTCTGGGACTGAGGAGCATTCTTTACCTG(+ATATAT)GTACCATCAATTGACAGGGAGGAATAGATTC NGG 10 8 17 8 24 0 100 5 10
    240606225344_e188 GCCCTGAATCTATTCCTCCCTGTCAATTGATG(+ATATAT)GTACCAGGTAAAGAATGCTCCTCAGTCCCAG NGG 10 8 17 8 24 0 100 5 10
    240606225342_z3d5 TAAAACCTAGTTTTAATACCTATTTCCTAGAG(+ATATAT)GATCCTAAATATAGTCATTGCAACTTTCCCC NGG 10 8 17 8 24 0 100 5 10
    240606225341_igsg CTTTAGGGGAAAGTTGCAATGACTATATTTAG(+ATATAT)GATCCTCTAGGAAATAGGTATTAAAACTAGG NGG 10 8 17 8 24 0 100 5 10
    240606225339_nc4t ATGTTCCAGTACCAATGGAAGAACTGCTATAG(+ATATAT)GTACCTGCCTAATAAGATTTCTAAACCCATA NGG 10 8 17 8 24 0 100 5 10
    240606225338_af67 TTAACTATGGGTTTAGAAATCTTATTAGGCAG(+ATATAT)GTACCTATAGCAGTTCTTCCATTGGTACTGG NGG 10 8 17 8 24 0 100 5 10
    240606225336_6w4c TTTGGCTTTCACTGGATGTGCCACGGGGTGGG(+ATATAT)GATCCTGTCTGATTCTGAATCAATTGGCCAG NGG 10 8 17 8 24 0 100 5 10
    240606225334_lbl4 TGCATCTGGCCAATTGATTCAGAATCAGACAG(+ATATAT)GATCCCCACCCCGTGGCACATCCAGTGAAAG NGG 10 8 17 8 24 0 100 5 10