Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606225332_sriz AATGTTTATTCCACCTTTCTGTCATATTCCTG(+ATATAT)GATCCTTCACCAATGTTACACGAGGAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240606225330_l796 TTTGTTTTTTTCCTCGTGTAACATTGGTGAAG(+ATATAT)GATCCAGGAATATGACAGAAAGGTGGAATAA NGG 10 8 17 8 24 0 100 5 10
    240606225328_82yt CTGCTTTGACAGGATATCTGCTCTCAGAAACG(+ATATAT)GATCCATAACTCGTTAGTGAGTGACTCCTAG NGG 10 8 17 8 24 0 100 5 10
    240606225326_1b1i CCTAACTAGGAGTCACTCACTAACGAGTTATG(+ATATAT)GATCCGTTTCTGAGAGCAGATATCCTGTCAA NGG 10 8 17 8 24 0 100 5 10
    240606225324_97w4 TTTCTAGACACAGTTGACTGTCAATGAACTGG(+ATATAT)GATCCTACAAGATGACAAGATGTTCAACTCA NGG 10 8 17 8 24 0 100 5 10
    240606225322_9fgp GTGTGTGAGTTGAACATCTTGTCATCTTGTAG(+ATATAT)GATCCCAGTTCATTGACAGTCAACTGTGTCT NGG 10 8 17 8 24 0 100 5 10
    240606225320_v51w ATGTGAGGTAATAAATCATCCAGACAGGCTCG(+ATATAT)GATCCAATCAGATAACATTCCAAGTTGCTCC NGG 10 8 17 8 24 0 100 5 10
    240606225318_z4ca TGCCAGGAGCAACTTGGAATGTTATCTGATTG(+ATATAT)GATCCGAGCCTGTCTGGATGATTTATTACCT NGG 10 8 17 8 24 0 100 5 10
    240606225315_hi74 ACAGCCCTAGGTAAACATCCATCTAACCAAGG(+ATATAT)GATCCAAAAGCTAACTGTCCGTGCTGGGTCT NGG 10 8 17 8 24 0 100 5 10
    240606225314_bfj9 AGAGGAGACCCAGCACGGACAGTTAGCTTTTG(+ATATAT)GATCCCTTGGTTAGATGGATGTTTACCTAGG NGG 10 8 17 8 24 0 100 5 10