Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606225312_bjp8 AAGGATCAGCTGTATGAGACTGGTCCTATGAG(+ATATAT)GTACCCAAACATCATTAAAGGGCAACTTCTG NGG 10 8 17 8 24 0 100 5 10
    240606225310_a537 GGAAACAGAAGTTGCCCTTTAATGATGTTTGG(+ATATAT)GTACCTCATAGGACCAGTCTCATACAGCTGA NGG 10 8 17 8 24 0 100 5 10
    240606225308_g49d ACAAACACACAAATAATAATACAAATCCAGTG(+ATATAT)GATCCTTCACGTCTAATGATAAACTGAGAAC NGG 10 8 17 8 24 0 100 5 10
    240606225306_biml ATGTAGTTCTCAGTTTATCATTAGACGTGAAG(+ATATAT)GATCCACTGGATTTGTATTATTATTTGTGTG NGG 10 8 17 8 24 0 100 5 10
    240606225304_y8tr ACTGGCCCAGTCTTGTGAAGACTAGCTCCGTG(+ATATAT)GTACCTCCCCAAGTTAGAAGACGTGCTCATT NGG 10 8 17 8 24 0 100 5 10
    240606225301_5mtz GTTCCAATGAGCACGTCTTCTAACTTGGGGAG(+ATATAT)GTACCACGGAGCTAGTCTTCACAAGACTGGG NGG 10 8 17 8 24 0 100 5 10
    240606225259_ud10 TTGGTGGCACAAAGCAGCAATGTGACCTAGTG(+ATATAT)GATCCGAAAGGGACGGGATCTGAAATTACCC NGG 10 8 17 8 24 0 100 5 10
    240606225258_hzcr GAGAAGGGTAATTTCAGATCCCGTCCCTTTCG(+ATATAT)GATCCACTAGGTCACATTGCTGCTTTGTGCC NGG 10 8 17 8 24 0 100 5 10
    240606225256_degr AACTTACATATTTATTGCCCAGGTGTAGGGAG(+ATATAT)GATCCATCACGATGTCAGTTCTTGGTTTTCC NGG 10 8 17 8 24 0 100 5 10
    240606225254_vd0a ACAGGGGAAAACCAAGAACTGACATCGTGATG(+ATATAT)GATCCTCCCTACACCTGGGCAATAAATATGT NGG 10 8 17 8 24 0 100 5 10