Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606225638_ni7m ACAAAGTCCTTTAATTTAAAAAAAAAAAAAAG(+ATATAT)GTACCTGATTCTCACTTCTCTCAATCTTCTA NGG 10 8 17 8 24 0 100 5 10
    240606225637_g2v1 GTAAATAGAAGATTGAGAGAAGTGAGAATCAG(+ATATAT)GTACCTTTTTTTTTTTTTTAAATTAAAGGAC NGG 10 8 17 8 24 0 100 5 10
    240606225635_naxz GTTACAGATTGCTCTTTATTATAATACCAGTG(+ATATAT)GTACCCAAAGAAAAAAGCAGAGCATTATGTA NGG 10 8 17 8 24 0 100 5 10
    240606225633_flk1 AAACTTACATAATGCTCTGCTTTTTTCTTTGG(+ATATAT)GTACCACTGGTATTATAATAAAGAGCAATCT NGG 10 8 17 8 24 0 100 5 10
    240606225631_j4fk TCACATCACTGGTGTGAAGTGATGTGAAAAGG(+ATATAT)GTACCTTCATCGAGTGCTGTACCTGCTGTTC NGG 10 8 17 8 24 0 100 5 10
    240606225629_jckr GCACAGAACAGCAGGTACAGCACTCGATGAAG(+ATATAT)GTACCCTTTTCACATCACTTCACACCAGTGA NGG 10 8 17 8 24 0 100 5 10
    240606225627_via1 GCATCACAATTATTCATTTGTGGAACATTTTG(+ATATAT)GTACCTGAAATATTTACATAAGAAGGCGGAA NGG 10 8 17 8 24 0 100 5 10
    240606225626_a5yu TGGGTTTCCGCCTTCTTATGTAAATATTTCAG(+ATATAT)GTACCAAAATGTTCCACAAATGAATAATTGT NGG 10 8 17 8 24 0 100 5 10
    240606225624_42r3 TCGCTTGGAGGGTGGACAAGGGGCAGTCCTAG(+ATATAT)GTACCAGGCTCTCAGTCTCGAGCATCCCAGA NGG 10 8 17 8 24 0 100 5 10
    240606225622_tk40 CGGTGTCTGGGATGCTCGAGACTGAGAGCCTG(+ATATAT)GTACCTAGGACTGCCCCTTGTCCACCCTCCA NGG 10 8 17 8 24 0 100 5 10