Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606225017_hktf GAAGCATTAGATCCTGTCCTGAAGGAGTCTGG(+ATATAT)GTACCCACTTAGTGTCCTCTGGTCAAAATGT NGG 10 8 17 8 24 0 100 5 10
    240606225015_949h TAGAAACATTTTGACCAGAGGACACTAAGTGG(+ATATAT)GTACCCAGACTCCTTCAGGACAGGATCTAAT NGG 10 8 17 8 24 0 100 5 10
    240606225014_fzsf CTACTTAAAAAAGAAACACTTTCAGGTTATAG(+ATATAT)GTACCTGCAGAAGTGTTCAGGAACAAACAAT NGG 10 8 17 8 24 0 100 5 10
    240606225013_jw8n TTATGATTGTTTGTTCCTGAACACTTCTGCAG(+ATATAT)GTACCTATAACCTGAAAGTGTTTCTTTTTTA NGG 10 8 17 8 24 0 100 5 10
    240606225011_5185 AGCAGCAGCTGTACACTTCTGGAATGACACAG(+ATATAT)GTACCAACACAGAGTTCATGGCCCCAGGAAC NGG 10 8 17 8 24 0 100 5 10
    240606225009_mt8l ATGTGGTTCCTGGGGCCATGAACTCTGTGTTG(+ATATAT)GTACCTGTGTCATTCCAGAAGTGTACAGCTG NGG 10 8 17 8 24 0 100 5 10
    240606225007_03my ATAGGCCTGTCACTGCAAAACCACCGTCCCTG(+ATATAT)GTACCAACTTTTGTCTTAGTTACGATTTTAT NGG 10 8 17 8 24 0 100 5 10
    240606225005_k4i7 CAGCAATAAAATCGTAACTAAGACAAAAGTTG(+ATATAT)GTACCAGGGACGGTGGTTTTGCAGTGACAGG NGG 10 8 17 8 24 0 100 5 10
    240606225003_gdc7 ATGGGAGAGAGTGACCGAGCTAGGAACAGAGG(+ATATAT)GATCCCAGCCACTCCAAGGTAGAGTCTGTGT NGG 10 8 17 8 24 0 100 5 10
    240606225001_ep9a TCCATACACAGACTCTACCTTGGAGTGGCTGG(+ATATAT)GATCCCTCTGTTCCTAGCTCGGTCACTCTCT NGG 10 8 17 8 24 0 100 5 10