Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606224933_dr5s GGATGGACCATGTAGAGACTGCCATATCCAGG(+ATATAT)GATCCACCCCATAATCAGCATCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240606224931_yqeu GGTGTCAGCGTTTGGATGCTGATTATGGGGTG(+ATATAT)GATCCCTGGATATGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240606224929_x78s CTGTGCTGCCAGCCCCTGCCCAGGTTCCTTTG(+ATATAT)GATCCGCAGATGAAAGACACACACACACACA NGG 10 8 17 8 24 0 100 5 10
    240606224927_1wns GTGTGTGTGTGTGTGTGTGTCTTTCATCTGCG(+ATATAT)GATCCAAAGGAACCTGGGCAGGGGCTGGCAG NGG 10 8 17 8 24 0 100 5 10
    240606224926_c8j6 TTAATTTCACAGATTCTTGTTCCAATGTCTAG(+ATATAT)GATCCATACATGATCTTTAGGGCTCCTTCAA NGG 10 8 17 8 24 0 100 5 10
    240606224924_vjfa GCCCTTTGAAGGAGCCCTAAAGATCATGTATG(+ATATAT)GATCCTAGACATTGGAACAAGAATCTGTGAA NGG 10 8 17 8 24 0 100 5 10
    240606224923_rsw9 TTTAGATGATAAATTTCTCATGTCAATTTTTG(+ATATAT)GTACCATTATTATCTATATTTAATAGATAAG NGG 10 8 17 8 24 0 100 5 10
    240606224921_6ik5 TTTCTCTTATCTATTAAATATAGATAATAATG(+ATATAT)GTACCAAAAATTGACATGAGAAATTTATCAT NGG 10 8 17 8 24 0 100 5 10
    240606224920_mql3 CTAAATTTGTAAATGGCAGCAGTATTATAAAG(+ATATAT)GATCCAAATGACCTAACAGGAACCAGAAAAA NGG 10 8 17 8 24 0 100 5 10
    240606224918_y5md CAATTTTTTTCTGGTTCCTGTTAGGTCATTTG(+ATATAT)GATCCTTTATAATACTGCTGCCATTTACAAA NGG 10 8 17 8 24 0 100 5 10