Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606224832_fay4 GCACTGCCAGACTTACTTACTAAGCCTGCTGG(+ATATAT)GATCCCAGGCCTGCCCTGGGACAGAGAGGCC NGG 10 8 17 8 24 0 100 5 10
    240606224830_p9vd TTCTCGGCCTCTCTGTCCCAGGGCAGGCCTGG(+ATATAT)GATCCCAGCAGGCTTAGTAAGTAAGTCTGGC NGG 10 8 17 8 24 0 100 5 10
    240606224828_tnpp AAAACTGTAGCTTACTCATCTTAGTAGGTTAG(+ATATAT)GTACCAAAGTCCAGGCTGTTTATTTCTAACC NGG 10 8 17 8 24 0 100 5 10
    240606224826_mh4u ATTTGGGTTAGAAATAAACAGCCTGGACTTTG(+ATATAT)GTACCTAACCTACTAAGATGAGTAAGCTACA NGG 10 8 17 8 24 0 100 5 10
    240606224824_65qq TGTCATCCCTCTTTGGGCCCGAAGGCCAGAGG(+ATATAT)GTACCAACTGGGCAAATGCCATCTGCCTTTT NGG 10 8 17 8 24 0 100 5 10
    240606224823_rgz0 TGGGTAAAAGGCAGATGGCATTTGCCCAGTTG(+ATATAT)GTACCCTCTGGCCTTCGGGCCCAAAGAGGGA NGG 10 8 17 8 24 0 100 5 10
    240606224821_zqs2 CCTAGGTTATGAATGACAGCCACAGATGCTGG(+ATATAT)GATCCCTATTGAGAAGGCAGTGCTATCGAGA NGG 10 8 17 8 24 0 100 5 10
    240606224819_zo4y CGGGCTCTCGATAGCACTGCCTTCTCAATAGG(+ATATAT)GATCCCAGCATCTGTGGCTGTCATTCATAAC NGG 10 8 17 8 24 0 100 5 10
    240606224816_mgu8 GGTTGCTCCGTGCATAACTAGTTCTTTCTGGG(+ATATAT)GATCCTGTCTGTCTCTTTTTTCCCCTGACCT NGG 10 8 17 8 24 0 100 5 10
    240606224815_lqk9 GACAGAGGTCAGGGGAAAAAAGAGACAGACAG(+ATATAT)GATCCCCAGAAAGAACTAGTTATGCACGGAG NGG 10 8 17 8 24 0 100 5 10