Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606224717_x5u8 AAGGAGTTTTACTCCTTGGTTTCAAAGCAGAG(+ATATAT)GATCCGCCTATGGACAGGTTCATGAAAATGA NGG 10 8 17 8 24 0 100 5 10
    240606224715_suij ATCCATCATTTTCATGAACCTGTCCATAGGCG(+ATATAT)GATCCTCTGCTTTGAAACCAAGGAGTAAAAC NGG 10 8 17 8 24 0 100 5 10
    240606224713_2t30 GGTATCATCTCCAGTTTCAGGCATCCAGTGGG(+ATATAT)GATCCCTGACAGGTGGGAGAGAACTACTATA NGG 10 8 17 8 24 0 100 5 10
    240606224711_tlyw ATTATTATAGTAGTTCTCTCCCACCTGTCAGG(+ATATAT)GATCCCCACTGGATGCCTGAAACTGGAGATG NGG 10 8 17 8 24 0 100 5 10
    240606224710_9i0o AAAGGATAACTTTTCTTTCTTTTGACTATGTG(+ATATAT)GATCCTACATATTGAACTCAGATAGTCAGGG NGG 10 8 17 8 24 0 100 5 10
    240606224708_fhjf GCTAACCCTGACTATCTGAGTTCAATATGTAG(+ATATAT)GATCCACATAGTCAAAAGAAAGAAAAGTTAT NGG 10 8 17 8 24 0 100 5 10
    240606224706_bb6w GGGGGGGGGCGGGGGTGGTGTGGTCTGAACAG(+ATATAT)GATCCCACAGTAGCTCAGGCTGGCCTTGAAC NGG 10 8 17 8 24 0 100 5 10
    240606224704_sjny AGAAAGTTCAAGGCCAGCCTGAGCTACTGTGG(+ATATAT)GATCCTGTTCAGACCACACCACCCCCGCCCC NGG 10 8 17 8 24 0 100 5 10
    240606224703_3q8v ATGGAATCTTATTCCAACACAACTTCCAAATG(+ATATAT)GATCCTCTCCTCAAAATAATCTGGTATGAAG NGG 10 8 17 8 24 0 100 5 10
    240606224701_sl1p AGTTTCTTCATACCAGATTATTTTGAGGAGAG(+ATATAT)GATCCATTTGGAAGTTGTGTTGGAATAAGAT NGG 10 8 17 8 24 0 100 5 10