Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606224636_p47t TCCTGGCAGAGCTCCACACGGCCTTCCTGGTG(+ATATAT)GATCCTAAAGGAGGGCGCATAGCAAGGTGCT NGG 10 8 17 8 24 0 100 5 10
    240606224634_k5w6 TCCGGAGCACCTTGCTATGCGCCCTCCTTTAG(+ATATAT)GATCCACCAGGAAGGCCGTGTGGAGCTCTGC NGG 10 8 17 8 24 0 100 5 10
    240606224633_to72 ATGTCACAGGGCTGTGTCCACACCAGCAGCAG(+ATATAT)GATCCTTGGGTACCCTTTGCTGCTTCCTTTC NGG 10 8 17 8 24 0 100 5 10
    240606224630_hjz1 GAAGGGAAAGGAAGCAGCAAAGGGTACCCAAG(+ATATAT)GATCCTGCTGCTGGTGTGGACACAGCCCTGT NGG 10 8 17 8 24 0 100 5 10
    240606224628_59wc TCTTCTGAGATAATAAGCACAATCCTGAAATG(+ATATAT)GATCCAATAATTCAGCTAGATATTATAGATT NGG 10 8 17 8 24 0 100 5 10
    240606224627_klxu ATACTAATCTATAATATCTAGCTGAATTATTG(+ATATAT)GATCCATTTCAGGATTGTGCTTATTATCTCA NGG 10 8 17 8 24 0 100 5 10
    240606224625_k3sp ACTTCTTTTCTTCCTCTCCTCTAAGTGAACAG(+ATATAT)GATCCCCTTTTTGGTCCATTTGTCCATGCCT NGG 10 8 17 8 24 0 100 5 10
    240606224623_voic GTGAGAGGCATGGACAAATGGACCAAAAAGGG(+ATATAT)GATCCTGTTCACTTAGAGGAGAGGAAGAAAA NGG 10 8 17 8 24 0 100 5 10
    240606224622_j3mp TTGAGAATATAACAAAACAGCACTTTCTTTTG(+ATATAT)GATCCTCACCCATTGCCTGGTGATAATGCTA NGG 10 8 17 8 24 0 100 5 10
    240606224621_833g CAGCCTAGCATTATCACCAGGCAATGGGTGAG(+ATATAT)GATCCAAAAGAAAGTGCTGTTTTGTTATATT NGG 10 8 17 8 24 0 100 5 10