Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606224529_axl7 ACACGGCCCACACCTAGCAGACTCCTCGGAAG(+ATATAT)GATCCATAACAGCCTGGCCCAATGACCTGGA NGG 10 8 17 8 24 0 100 5 10
    240606224528_1qtu GGACTTCCAGGTCATTGGGCCAGGCTGTTATG(+ATATAT)GATCCTTCCGAGGAGTCTGCTAGGTGTGGGC NGG 10 8 17 8 24 0 100 5 10
    240606224527_axds GTTGTGAGAGAAACTCTTCCACAACATTATGG(+ATATAT)GATCCAAAAGGAGAGGACCCAAGGGGCTTAA NGG 10 8 17 8 24 0 100 5 10
    240606224526_ycw1 CTCTTTTAAGCCCCTTGGGTCCTCTCCTTTTG(+ATATAT)GATCCCATAATGTTGTGGAAGAGTTTCTCTC NGG 10 8 17 8 24 0 100 5 10
    240606224524_z1kb GGCATTCAGAGTTGGTCATTGTGGGTAAGAGG(+ATATAT)GTACCGGCCCACCCGAAAGCCAGCATCCACG NGG 10 8 17 8 24 0 100 5 10
    240606224522_68gi CTGCTCGTGGATGCTGGCTTTCGGGTGGGCCG(+ATATAT)GTACCCTCTTACCCACAATGACCAACTCTGA NGG 10 8 17 8 24 0 100 5 10
    240606224520_mov0 TCCCCTGAGTACTGAGGCTCACCACCCAGCTG(+ATATAT)GTACCCTCGCCCTGCCTTCTGCCTCTGAAGG NGG 10 8 17 8 24 0 100 5 10
    240606224519_xsqs TAAATCCTTCAGAGGCAGAAGGCAGGGCGAGG(+ATATAT)GTACCAGCTGGGTGGTGAGCCTCAGTACTCA NGG 10 8 17 8 24 0 100 5 10
    240606224517_pup5 TTCATACATTTAGAAGGCATATAAAGAGATAG(+ATATAT)GATCCCTTTAGCCCAGGCTGATCTTGAACTT NGG 10 8 17 8 24 0 100 5 10
    240606224516_mxl4 ACTACAAGTTCAAGATCAGCCTGGGCTAAAGG(+ATATAT)GATCCTATCTCTTTATATGCCTTCTAAATGT NGG 10 8 17 8 24 0 100 5 10