Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606224438_6pxr TCCTCAGAAAATTGGACATAGTACTACCGGAG(+ATATAT)GATCCAGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240606224437_z4ky TTCTGGATATATGCCCAGGAGAGGTATTGCTG(+ATATAT)GATCCTCCGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240606224435_aoli GGATGGACCATGTAGAGACTGCCATATCCAGG(+ATATAT)GATCCACCCCATAATCAGCTTCCAAATGCTG NGG 10 8 17 8 24 0 100 5 10
    240606224433_uue8 GGTGTCAGCATTTGGAAGCTGATTATGGGGTG(+ATATAT)GATCCCTGGATATGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240606224432_s80s AGAAGCATTCCTTGTCCTAGCCCTTCTCATTG(+ATATAT)GTACCCTTGACTCTAAGCAGGGTCCCAAAGA NGG 10 8 17 8 24 0 100 5 10
    240606224430_vwcq AAGCCTCTTTGGGACCCTGCTTAGAGTCAAGG(+ATATAT)GTACCAATGAGAAGGGCTAGGACAAGGAATG NGG 10 8 17 8 24 0 100 5 10
    240606224427_puup TGACCACTGAGATATGGATGATGGACTCCAAG(+ATATAT)GATCCTTCAGCGGTATCTATTGTTGTTCTCT NGG 10 8 17 8 24 0 100 5 10
    240606224426_qo0t AAAACAGAGAACAACAATAGATACCGCTGAAG(+ATATAT)GATCCTTGGAGTCCATCATCCATATCTCAGT NGG 10 8 17 8 24 0 100 5 10
    240606224425_640d GATTGAGCTCCAGCTATTCAGCTTGGCAGCAG(+ATATAT)GTACCTTGAGCCCGAGCCACCTTGCTAGCAC NGG 10 8 17 8 24 0 100 5 10
    240606224423_5prk GACTTGTGCTAGCAAGGTGGCTCGGGCTCAAG(+ATATAT)GTACCTGCTGCCAAGCTGAATAGCTGGAGCT NGG 10 8 17 8 24 0 100 5 10