Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606224406_9dwf GCATATAAGCCCCCCTGTTCTATGAGATCTGG(+ATATAT)GTACCCTCACTGCCTTCCCAGCTCCGAGTCT NGG 10 8 17 8 24 0 100 5 10
    240606224404_g0i4 CATGGAGACTCGGAGCTGGGAAGGCAGTGAGG(+ATATAT)GTACCCAGATCTCATAGAACAGGGGGGCTTA NGG 10 8 17 8 24 0 100 5 10
    240606224401_bh29 AAAATGTCCTTGATAATAAATGGTATGGCCCG(+ATATAT)GATCCTATTTTGATAAGATCCAGGGGAGCTA NGG 10 8 17 8 24 0 100 5 10
    240606224400_w5ev ACAGATAGCTCCCCTGGATCTTATCAAAATAG(+ATATAT)GATCCGGGCCATACCATTTATTATCAAGGAC NGG 10 8 17 8 24 0 100 5 10
    240606224358_uty4 GCTATATAGCATTGATCAAATACTTAAATAAG(+ATATAT)GATCCCAAGCAAGATCTATAGATTGTTGGGA NGG 10 8 17 8 24 0 100 5 10
    240606224356_3gz8 GTGCATCCCAACAATCTATAGATCTTGCTTGG(+ATATAT)GATCCTTATTTAAGTATTTGATCAATGCTAT NGG 10 8 17 8 24 0 100 5 10
    240606224355_22wn TAATAAGTCTCGGTGTCATCATTTAACTGGAG(+ATATAT)GATCCAGAAAGTCCCACTACAATGTATTGCA NGG 10 8 17 8 24 0 100 5 10
    240606224352_r95k CTATCTGCAATACATTGTAGTGGGACTTTCTG(+ATATAT)GATCCTCCAGTTAAATGATGACACCGAGACT NGG 10 8 17 8 24 0 100 5 10
    240606224351_6r41 GACAGATCCAGTTCAAAATCCTAGAGGCAGTG(+ATATAT)GATCCTACAAGAGACTTGTGATTTTTTTTCT NGG 10 8 17 8 24 0 100 5 10
    240606224350_d3ej AGGGAAGAAAAAAAATCACAAGTCTCTTGTAG(+ATATAT)GATCCACTGCCTCTAGGATTTTGAACTGGAT NGG 10 8 17 8 24 0 100 5 10