Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606224313_p75n GCAGGGGAGCTCAGAGCTTCAGCCCTGCCTAG(+ATATAT)GTACCACAGTGAAAACAGGCAATCCGTCCCC NGG 10 8 17 8 24 0 100 5 10
    240606224312_w2e9 GGTGGGGGGACGGATTGCCTGTTTTCACTGTG(+ATATAT)GTACCTAGGCAGGGCTGAAGCTCTGAGCTCC NGG 10 8 17 8 24 0 100 5 10
    240606224310_lmhu GAGTATGTTGTAGCTATCTTCAGAGGGCATGG(+ATATAT)GATCCCATGGTGAGCCACCATGTGGGTGCTG NGG 10 8 17 8 24 0 100 5 10
    240606224308_38c9 ATTCCCAGCACCCACATGGTGGCTCACCATGG(+ATATAT)GATCCCATGCCCTCTGAAGATAGCTACAACA NGG 10 8 17 8 24 0 100 5 10
    240606224307_hl17 GCAACTCTTAAATAAAGCAAATATACAGACTG(+ATATAT)GATCCGGCTGAGACAAGCTGGGTAATCAGAC NGG 10 8 17 8 24 0 100 5 10
    240606224305_tecr CAAATGTCTGATTACCCAGCTTGTCTCAGCCG(+ATATAT)GATCCAGTCTGTATATTTGCTTTATTTAAGA NGG 10 8 17 8 24 0 100 5 10
    240606224303_a0w5 AGATCATGTCTCCACTTACTGTCACTCCTAAG(+ATATAT)GATCCCCTGAAGGTCTTGGAGCTTCACATCC NGG 10 8 17 8 24 0 100 5 10
    240606224301_5u0q TCCAGGGATGTGAAGCTCCAAGACCTTCAGGG(+ATATAT)GATCCTTAGGAGTGACAGTAAGTGGAGACAT NGG 10 8 17 8 24 0 100 5 10
    240606224259_peqn CGATCTATGACAATGTTTGGAACTCTCTTTTG(+ATATAT)GATCCACAAAGCTCCTGCAATCAGCTCCAAT NGG 10 8 17 8 24 0 100 5 10
    240606224258_56ga GGGTTATTGGAGCTGATTGCAGGAGCTTTGTG(+ATATAT)GATCCAAAAGAGAGTTCCAAACATTGTCATA NGG 10 8 17 8 24 0 100 5 10