Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606230829_mikj ATCCAGAGCTTATGAAGAGAATGGTAAGACTG(+ATATAT)GTACCACATGGATGGGAGATGAAGTTGTCAC NGG 10 8 17 8 24 0 100 5 10
    240606230828_pdz7 TTACATTAACAAGGCAAAATAAAGCCACAATG(+ATATAT)GTACCATTAGGAAATTCCATCAAAACAACAG NGG 10 8 17 8 24 0 100 5 10
    240606230826_agql AAAGTCTGTTGTTTTGATGGAATTTCCTAATG(+ATATAT)GTACCATTGTGGCTTTATTTTGCCTTGTTAA NGG 10 8 17 8 24 0 100 5 10
    240606230824_ednz TGGTGTGATAGTTTCTTAGGAGCAAACAACAG(+ATATAT)GATCCTTTCTCCTAAACACAGGTTTAATTTT NGG 10 8 17 8 24 0 100 5 10
    240606230823_1808 TGTTGAAAATTAAACCTGTGTTTAGGAGAAAG(+ATATAT)GATCCTGTTGTTTGCTCCTAAGAAACTATCA NGG 10 8 17 8 24 0 100 5 10
    240606230820_cxv9 GCATGGGATGTCCTATTTTAGGAGGTTTCCAG(+ATATAT)GATCCTAGCCCACTAAATTAACATCATGCCA NGG 10 8 17 8 24 0 100 5 10
    240606230819_5sig AAGCCTGGCATGATGTTAATTTAGTGGGCTAG(+ATATAT)GATCCTGGAAACCTCCTAAAATAGGACATCC NGG 10 8 17 8 24 0 100 5 10
    240606230817_k28j CTAAGAGCATCATCTATTCTACTTGGGATAAG(+ATATAT)GTACCTGGTAAAGCTCTAAGCTCTAAAGTTA NGG 10 8 17 8 24 0 100 5 10
    240606230815_doic CCTTCTAACTTTAGAGCTTAGAGCTTTACCAG(+ATATAT)GTACCTTATCCCAAGTAGAATAGATGATGCT NGG 10 8 17 8 24 0 100 5 10
    240606230814_mkuu GAGACGCTCTTAGGGGGAGCACGACTTTCATG(+ATATAT)GATCCTGGGGCCTGGGTGGGCTGGGGGACAG NGG 10 8 17 8 24 0 100 5 10