Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606224121_jc1g TCAGCCTGAACCATTTCTCATCTGTTTCCCAG(+ATATAT)GATCCAAAATTGTACCTGCCTTTCAATAACT NGG 10 8 17 8 24 0 100 5 10
    240606224119_76fc GGAGGAGTTATTGAAAGGCAGGTACAATTTTG(+ATATAT)GATCCTGGGAAACAGATGAGAAATGGTTCAG NGG 10 8 17 8 24 0 100 5 10
    240606224118_3frr GAGGTCTTAAGATCCCATGGAGGGTCCTGTGG(+ATATAT)GTACCTTGTGGGGGTCCGCAGACTCTGTGCC NGG 10 8 17 8 24 0 100 5 10
    240606224116_x5j1 CCTTGGGCACAGAGTCTGCGGACCCCCACAAG(+ATATAT)GTACCCACAGGACCCTCCATGGGATCTTAAG NGG 10 8 17 8 24 0 100 5 10
    240606224114_g5f1 GGATGGACCATGTAGAGACTGCCATATCCAGG(+ATATAT)GATCCACCCCATAATCAGCTTCCAAATGCTG NGG 10 8 17 8 24 0 100 5 10
    240606224111_zc9f GGTGTCAGCATTTGGAAGCTGATTATGGGGTG(+ATATAT)GATCCCTGGATATGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240606224109_kacp CTTTAATGCCATCACTTGAGCAGAGACAGGTG(+ATATAT)GATCCCTAAGTTTAAGTCCAGCCTCATCTAC NGG 10 8 17 8 24 0 100 5 10
    240606224107_6s6s GTTCTGTAGATGAGGCTGGACTTAAACTTAGG(+ATATAT)GATCCACCTGTCTCTGCTCAAGTGATGGCAT NGG 10 8 17 8 24 0 100 5 10
    240606224105_if1n CTTTAATGCCATCACTTGAGCAGAGACAGGTG(+ATATAT)GATCCCTAAGTTTAAGTCCAGCCTCATCTAC NGG 10 8 17 8 24 0 100 5 10
    240606224103_u0g8 GTTCTGTAGATGAGGCTGGACTTAAACTTAGG(+ATATAT)GATCCACCTGTCTCTGCTCAAGTGATGGCAT NGG 10 8 17 8 24 0 100 5 10