Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606224026_6w2c TTTTCAGACCAGCGACTCAGTTCATACAATGG(+ATATAT)GATCCCAATTTTGTTGTAAAAATTTTTTGCC NGG 10 8 17 8 24 0 100 5 10
    240606224024_tqhc TTTTTGGCAAAAAATTTTTACAACAAAATTGG(+ATATAT)GATCCCATTGTATGAACTGAGTCGCTGGTCT NGG 10 8 17 8 24 0 100 5 10
    240606224022_46pi TCCTCCGTGGAGCTGTCTCTAGTGAGAATCAG(+ATATAT)GTACCTGATTTGGTGAACCTGGATGGGAACC NGG 10 8 17 8 24 0 100 5 10
    240606224021_lpn4 TCCCAGGTTCCCATCCAGGTTCACCAAATCAG(+ATATAT)GTACCTGATTCTCACTAGAGACAGCTCCACG NGG 10 8 17 8 24 0 100 5 10
    240606224019_8smp ATGGTGGACCGGCCACTGTGCCTATGCTATGG(+ATATAT)GATCCCATCTCTTACAGCCCTGCAGGGAAGC NGG 10 8 17 8 24 0 100 5 10
    240606224016_emh5 CAAGAGCTTCCCTGCAGGGCTGTAAGAGATGG(+ATATAT)GATCCCATAGCATAGGCACAGTGGCCGGTCC NGG 10 8 17 8 24 0 100 5 10
    240606224014_basm TGTGGACCCCTACTGGGACGTGCTTGGTTTGG(+ATATAT)GTACCTTCCTCGGTCCCACTGGCCGATTCTC NGG 10 8 17 8 24 0 100 5 10
    240606224012_iz9a AAGTTGAGAATCGGCCAGTGGGACCGAGGAAG(+ATATAT)GTACCCAAACCAAGCACGTCCCAGTAGGGGT NGG 10 8 17 8 24 0 100 5 10
    240606224010_o8rc CCACCTTGAAATCCCAAAGAAAGCAGTTCTTG(+ATATAT)GTACCATCTATTATGGTTCTACCATTAAAAA NGG 10 8 17 8 24 0 100 5 10
    240606224009_q0lt CCCCTTTTTTAATGGTAGAACCATAATAGATG(+ATATAT)GTACCAAGAACTGCTTTCTTTGGGATTTCAA NGG 10 8 17 8 24 0 100 5 10