Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606223916_sth4 ATTCTTTCACCAAGGAAGCAATGGTGGCCAGG(+ATATAT)GTACCAGTCAGAGCCATTATTGCTCAGAGAA NGG 10 8 17 8 24 0 100 5 10
    240606223915_9axk AGTAATTCTCTGAGCAATAATGGCTCTGACTG(+ATATAT)GTACCCTGGCCACCATTGCTTCCTTGGTGAA NGG 10 8 17 8 24 0 100 5 10
    240606223914_ux6s AGGAGTAATGTAAGGCTAGCTAAACTCTAGTG(+ATATAT)GTACCAACGGAAGACTTCCGGTACGCCAGGC NGG 10 8 17 8 24 0 100 5 10
    240606223912_wbmm ACCCCGCCTGGCGTACCGGAAGTCTTCCGTTG(+ATATAT)GTACCACTAGAGTTTAGCTAGCCTTACATTA NGG 10 8 17 8 24 0 100 5 10
    240606223910_ypw3 GTCCTTGGTTGTGAAGTATCATCCCAGTCATG(+ATATAT)GTACCCTGTGCTGGCAACAGAAAAAAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240606223908_mzvs TATTTTTTTTTTTTTTCTGTTGCCAGCACAGG(+ATATAT)GTACCATGACTGGGATGATACTTCACAACCA NGG 10 8 17 8 24 0 100 5 10
    240606223906_4a9w AGGCCTCACATCCCCACTCTGCTCCCCGCCTG(+ATATAT)GTACCTGTGTTAATTTAGACCTTCACCACCT NGG 10 8 17 8 24 0 100 5 10
    240606223905_izfv GAGGCAGGTGGTGAAGGTCTAAATTAACACAG(+ATATAT)GTACCAGGCGGGGAGCAGAGTGGGGATGTGA NGG 10 8 17 8 24 0 100 5 10
    240606223902_ri1n ATAAGTCCCCATTTGAGGTCTAGGTTCCCAGG(+ATATAT)GATCCATTTACCTGGCCAGCCAGAAGCCCCT NGG 10 8 17 8 24 0 100 5 10
    240606223900_wnla TATGCAGGGGCTTCTGGCTGGCCAGGTAAATG(+ATATAT)GATCCCTGGGAACCTAGACCTCAAATGGGGA NGG 10 8 17 8 24 0 100 5 10