Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606223858_w0o2 CAATAAACTTTCTTGAATCTTCGAGTCTCCTG(+ATATAT)GATCCCCATGGCCTCCATCACATCTTCTTCT NGG 10 8 17 8 24 0 100 5 10
    240606223856_u1h2 ACATTAGAAGAAGATGTGATGGAGGCCATGGG(+ATATAT)GATCCAGGAGACTCGAAGATTCAAGAAAGTT NGG 10 8 17 8 24 0 100 5 10
    240606223855_qbda TTAGATCGATTTTTTTTAAAAGTATGTGTTGG(+ATATAT)GTACCTTTTCTGTGTGTATGCTCAGGCAGGC NGG 10 8 17 8 24 0 100 5 10
    240606223854_7ll2 ACACAGCCTGCCTGAGCATACACACAGAAAAG(+ATATAT)GTACCCAACACATACTTTTAAAAAAAATCGA NGG 10 8 17 8 24 0 100 5 10
    240606223852_zktp TCTTCTCCACGTACCCACAGAAGGCAGAAGTG(+ATATAT)GTACCAGGCAAGAAGATGGGATTGTTGCATT NGG 10 8 17 8 24 0 100 5 10
    240606223850_pi41 AACAAAATGCAACAATCCCATCTTCTTGCCTG(+ATATAT)GTACCACTTCTGCCTTCTGTGGGTACGTGGA NGG 10 8 17 8 24 0 100 5 10
    240606223848_d9p4 ATGGTATATAAAGAGTGTACCTTATAAGCCAG(+ATATAT)GTACCTTTAATCCCAGAACTTGGGTTTGAAG NGG 10 8 17 8 24 0 100 5 10
    240606223847_e77e GCTGGCTTCAAACCCAAGTTCTGGGATTAAAG(+ATATAT)GTACCTGGCTTATAAGGTACACTCTTTATAT NGG 10 8 17 8 24 0 100 5 10
    240606223845_oiju GAATAAAAGAATAATATATAAAATTAATAAAG(+ATATAT)GTACCAAATGCATCTATAAAAAATGGTTCCT NGG 10 8 17 8 24 0 100 5 10
    240606223844_f0of TCTCAAGGAACCATTTTTTATAGATGCATTTG(+ATATAT)GTACCTTTATTAATTTTATATATTATTCTTT NGG 10 8 17 8 24 0 100 5 10