Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606223806_r3tb GGCAACTAGCTTACAGTGAAGAGGAGGCTGAG(+ATATAT)GATCCAAGCTCAGGCACTCTAGGCTACAAAA NGG 10 8 17 8 24 0 100 5 10
    240606223804_kn1w ACCCCTTTTGTAGCCTAGAGTGCCTGAGCTTG(+ATATAT)GATCCTCAGCCTCCTCTTCACTGTAAGCTAG NGG 10 8 17 8 24 0 100 5 10
    240606223803_gx5l CCCTGCCTTCACTGTAAAAGATCCCTAGGCAG(+ATATAT)GATCCTCTCACTACATATCCCCAAACTATAG NGG 10 8 17 8 24 0 100 5 10
    240606223801_unaa GAGTCCTATAGTTTGGGGATATGTAGTGAGAG(+ATATAT)GATCCTGCCTAGGGATCTTTTACAGTGAAGG NGG 10 8 17 8 24 0 100 5 10
    240606223758_099k GGATGGACCATCTAGAGACTGCCATATCCAGG(+ATATAT)GATCCATCCCATAATTAGCCTCCAAAGGATG NGG 10 8 17 8 24 0 100 5 10
    240606223756_wvbf GGTGTCATCCTTTGGAGGCTAATTATGGGATG(+ATATAT)GATCCCTGGATATGGCAGTCTCTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240606223755_sb9j ATTAGTAAGTCTAAAACACAAATAATTATAAG(+ATATAT)GATCCGGAGTTTATATCTTTTCAAGAGCTAT NGG 10 8 17 8 24 0 100 5 10
    240606223753_j6i9 TACAGATAGCTCTTGAAAAGATATAAACTCCG(+ATATAT)GATCCTTATAATTATTTGTGTTTTAGACTTA NGG 10 8 17 8 24 0 100 5 10
    240606223751_c65k TTCTGGATATATGCCCAGGAGAGGTATTGTGG(+ATATAT)GATCCTCCAGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240606223749_j3g9 TCCTTAGAAAATTGGACATAGTACTACTGGAG(+ATATAT)GATCCCACAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10