Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606223700_qq8x ATAGTTTTATGAATATAGCTTTTATTTCAAAG(+ATATAT)GTACCCAGCCAAAGATAATTAAAACTCTATT NGG 10 8 17 8 24 0 100 5 10
    240606223658_ybww AACAAAATAGAGTTTTAATTATCTTTGGCTGG(+ATATAT)GTACCTTTGAAATAAAAGCTATATTCATAAA NGG 10 8 17 8 24 0 100 5 10
    240606223657_b8dp AACCACAGCTCTGCTAGTCAGGACAACGTTAG(+ATATAT)GTACCACTGACGACCCACAGGCCACTCATGC NGG 10 8 17 8 24 0 100 5 10
    240606223656_8284 ACTCGGCATGAGTGGCCTGTGGGTCGTCAGTG(+ATATAT)GTACCTAACGTTGTCCTGACTAGCAGAGCTG NGG 10 8 17 8 24 0 100 5 10
    240606223654_0og6 GTGATCTAAAGACACTAATGCATAGTCCTTAG(+ATATAT)GATCCCCTAAGAAAAAGACCCCAGTTAAAAT NGG 10 8 17 8 24 0 100 5 10
    240606223652_aw4j CCTGTATTTTAACTGGGGTCTTTTTCTTAGGG(+ATATAT)GATCCTAAGGACTATGCATTAGTGTCTTTAG NGG 10 8 17 8 24 0 100 5 10
    240606223649_71yg CAAAATGTTTGATAATTATTCCAGATAAAACG(+ATATAT)GATCCTCAGAGAGCAGGTCACCACCACTCCT NGG 10 8 17 8 24 0 100 5 10
    240606223648_smt7 TACATAGGAGTGGTGGTGACCTGCTCTCTGAG(+ATATAT)GATCCGTTTTATCTGGAATAATTATCAAACA NGG 10 8 17 8 24 0 100 5 10
    240606223646_nmat TTGTTTGCTGTTTTCCATTTCCTGGCTCCATG(+ATATAT)GTACCATTGTTTGCCAGGGAGCCCCACATGT NGG 10 8 17 8 24 0 100 5 10
    240606223645_ig3h GGGCCACATGTGGGGCTCCCTGGCAAACAATG(+ATATAT)GTACCATGGAGCCAGGAAATGGAAAACAGCA NGG 10 8 17 8 24 0 100 5 10