Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606223626_hvc2 TTCTGTACTGCTTAAGATTCAGCAGGGCACTG(+ATATAT)GATCCCTGGGAGCTGGAGTTAGGTGACTATG NGG 10 8 17 8 24 0 100 5 10
    240606223623_m1v2 CCACTCATAGTCACCTAACTCCAGCTCCCAGG(+ATATAT)GATCCAGTGCCCTGCTGAATCTTAAGCAGTA NGG 10 8 17 8 24 0 100 5 10
    240606223622_yvvk GCATCAAGTCTACCTTTTGCATTGGTTTTATG(+ATATAT)GATCCATGTATATAGGTAGCCATCATAAACT NGG 10 8 17 8 24 0 100 5 10
    240606223620_w8ta CCTTCAGTTTATGATGGCTACCTATATACATG(+ATATAT)GATCCATAAAACCAATGCAAAAGGTAGACTT NGG 10 8 17 8 24 0 100 5 10
    240606223618_4ofq CACGTGACCCTGAACAACAGTGAGCCTCTCGG(+ATATAT)GATCCAGCCCACCATCACCTTGTTCGGAGCT NGG 10 8 17 8 24 0 100 5 10
    240606223617_zguv CTCATAGCTCCGAACAAGGTGATGGTGGGCTG(+ATATAT)GATCCCGAGAGGCTCACTGTTGTTCAGGGTC NGG 10 8 17 8 24 0 100 5 10
    240606223615_8aav TGTTATTTCTTTAGCAGTGGTTAGGATGCTAG(+ATATAT)GTACCACTGGTTTCTCTCATCAACGCTGCAG NGG 10 8 17 8 24 0 100 5 10
    240606223613_iapu TTTGGCTGCAGCGTTGATGAGAGAAACCAGTG(+ATATAT)GTACCTAGCATCCTAACCACTGCTAAAGAAA NGG 10 8 17 8 24 0 100 5 10
    240606223611_r8jr TTAACTACATCTACAGCTATGTTTGTTTCCCG(+ATATAT)GTACCCGGTTACAGTGAGTCTCTGCCCTGCT NGG 10 8 17 8 24 0 100 5 10
    240606223609_92qf TTCACAGCAGGGCAGAGACTCACTGTAACCGG(+ATATAT)GTACCGGGAAACAAACATAGCTGTAGATGTA NGG 10 8 17 8 24 0 100 5 10