Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606223607_y95z GCAGATGTTTTTGAGCTGCCATGTGATGACAG(+ATATAT)GATCCAAACCAGAGTTCTCTGCAAGAGCGGC NGG 10 8 17 8 24 0 100 5 10
    240606223606_84vm CACTTGCCGCTCTTGCAGAGAACTCTGGTTTG(+ATATAT)GATCCTGTCATCACATGGCAGCTCAAAAACA NGG 10 8 17 8 24 0 100 5 10
    240606223605_kt66 TCTGTACCTAGGGTCTGAACTTAGTTAATGAG(+ATATAT)GATCCCAGCAAGACTCTCCAGTTTACATTCC NGG 10 8 17 8 24 0 100 5 10
    240606223603_d2im GGTGAGGAATGTAAACTGGAGAGTCTTGCTGG(+ATATAT)GATCCTCATTAACTAAGTTCAGACCCTAGGT NGG 10 8 17 8 24 0 100 5 10
    240606223601_ln94 TTTTAGTACCGACATATATTATTTTCATTGTG(+ATATAT)GATCCATTTTTTATTGCATGTTCAGATGTCA NGG 10 8 17 8 24 0 100 5 10
    240606223600_m3rr GCAAATGACATCTGAACATGCAATAAAAAATG(+ATATAT)GATCCACAATGAAAATAATATATGTCGGTAC NGG 10 8 17 8 24 0 100 5 10
    240606223558_4yci GCTGTTTCAAGAGCATTAGGTACATGCACATG(+ATATAT)GTACCTTCCTAATTAATTGACCTGGAAAAAG NGG 10 8 17 8 24 0 100 5 10
    240606223556_juk5 CAGCTCTTTTTCCAGGTCAATTAATTAGGAAG(+ATATAT)GTACCATGTGCATGTACCTAATGCTCTTGAA NGG 10 8 17 8 24 0 100 5 10
    240606223555_oxqe ATAACTGTTAACTGCAACATATCTTTGGCAGG(+ATATAT)GATCCTCAGTCGTACATTTTGTCTTTAAATG NGG 10 8 17 8 24 0 100 5 10
    240606223553_pom8 AGAGGCATTTAAAGACAAAATGTACGACTGAG(+ATATAT)GATCCCTGCCAAAGATATGTTGCAGTTAACA NGG 10 8 17 8 24 0 100 5 10