Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606223537_twxa GGTTATAACTGTCTGTAACTCCAGTGTCAGTG(+ATATAT)GATCCAATATTCTCTTCTGGTTCCACAGGTG NGG 10 8 17 8 24 0 100 5 10
    240606223536_zx18 GTAGGCACCTGTGGAACCAGAAGAGAATATTG(+ATATAT)GATCCACTGACACTGGAGTTACAGACAGTTA NGG 10 8 17 8 24 0 100 5 10
    240606223534_mag4 CTTCTGGGAGACTTGCTGGTACTGACTTCCTG(+ATATAT)GTACCTAGAGTTACCATGGAATCGGTTTGTT NGG 10 8 17 8 24 0 100 5 10
    240606223533_v0xg ATAGAAACAAACCGATTCCATGGTAACTCTAG(+ATATAT)GTACCAGGAAGTCAGTACCAGCAAGTCTCCC NGG 10 8 17 8 24 0 100 5 10
    240606223531_l48g TTCTCATTCAGTAAAGGGAACTTGCTACAAAG(+ATATAT)GTACCCAAAACACACTTTCTGTTCTGCCGGT NGG 10 8 17 8 24 0 100 5 10
    240606223529_95l5 TGCTGACCGGCAGAACAGAAAGTGTGTTTTGG(+ATATAT)GTACCTTTGTAGCAAGTTCCCTTTACTGAAT NGG 10 8 17 8 24 0 100 5 10
    240606223528_uldi AAGGTTCTCACTGTCGTCAACAATCTTTCTAG(+ATATAT)GTACCTTTGGATGGTTCTAGCACATTTATCA NGG 10 8 17 8 24 0 100 5 10
    240606223526_05ur TTGTCTGATAAATGTGCTAGAACCATCCAAAG(+ATATAT)GTACCTAGAAAGATTGTTGACGACAGTGAGA NGG 10 8 17 8 24 0 100 5 10
    240606223524_91iu GAACCCCTGAACTTGGAAAACTGAGCCAAGAG(+ATATAT)GATCCCAAGTTCAAAGCCAGCCTGGGCTACA NGG 10 8 17 8 24 0 100 5 10
    240606223523_ived TGCTATGTAGCCCAGGCTGGCTTTGAACTTGG(+ATATAT)GATCCTCTTGGCTCAGTTTTCCAAGTTCAGG NGG 10 8 17 8 24 0 100 5 10