Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606223446_dpkx AGTGGCCAGAGGGAAGTGGATACGAGGGTAGG(+ATATAT)GTACCAGGTTGGTCTGGAATTCTGTCAGATC NGG 10 8 17 8 24 0 100 5 10
    240606223444_k2w3 ATGTTGATCTGACAGAATTCCAGACCAACCTG(+ATATAT)GTACCCTACCCTCGTATCCACTTCCCTCTGG NGG 10 8 17 8 24 0 100 5 10
    240606223443_rns9 AGGATTGGAAACACACTGCCTAGACAAAAATG(+ATATAT)GTACCCTGTGTTCTCTGCGTTCCTCACGTCT NGG 10 8 17 8 24 0 100 5 10
    240606223441_fx4i TAGCCAGACGTGAGGAACGCAGAGAACACAGG(+ATATAT)GTACCATTTTTGTCTAGGCAGTGTGTTTCCA NGG 10 8 17 8 24 0 100 5 10
    240606223439_zqfq AGTACTGGCAGTAGCCAGGGAAATCCCATTTG(+ATATAT)GATCCAAAGGCCAGCAGTTAGGAAAGAGGAA NGG 10 8 17 8 24 0 100 5 10
    240606223437_hlda TGACCTTCCTCTTTCCTAACTGCTGGCCTTTG(+ATATAT)GATCCAAATGGGATTTCCCTGGCTACTGCCA NGG 10 8 17 8 24 0 100 5 10
    240606223436_byt9 TCCAGTCAAATGTCTCATTGTTAGGTAGTGTG(+ATATAT)GTACCCAGACCTAGAGGGTCTGTAACCGGCC NGG 10 8 17 8 24 0 100 5 10
    240606223434_2v01 AGTCAGGCCGGTTACAGACCCTCTAGGTCTGG(+ATATAT)GTACCACACTACCTAACAATGAGACATTTGA NGG 10 8 17 8 24 0 100 5 10
    240606223431_iqs6 GTCAAGAAAGCGTGATGGCAAAGACGCCATGG(+ATATAT)GATCCCCTGACGTCAAGCAGACCTTGTTTGT NGG 10 8 17 8 24 0 100 5 10
    240606223428_4yq9 ATTCTACAAACAAGGTCTGCTTGACGTCAGGG(+ATATAT)GATCCCATGGCGTCTTTGCCATCACGCTTTC NGG 10 8 17 8 24 0 100 5 10