Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606225919_qm9p TGACTTTGCCTCAATAGTAAGCTTCATGAGAG(+ATATAT)GATCCTGCCAACTGTAAACAGGTTTTACCAA NGG 10 8 17 8 24 0 100 5 10
    240606225918_nrd2 TCCCACAAACATCTGTCTTGCCAGCTGGGCAG(+ATATAT)GATCCAATTCCCCATACTGGCTTGTATACCC NGG 10 8 17 8 24 0 100 5 10
    240606225916_d9wx TTTGAGGGTATACAAGCCAGTATGGGGAATTG(+ATATAT)GATCCTGCCCAGCTGGCAAGACAGATGTTTG NGG 10 8 17 8 24 0 100 5 10
    240606225914_od4l GTAAATGTATTTTTCCCTTTTAACTGTTCTTG(+ATATAT)GATCCAAAGCATATGACCACTGCTCATTGAC NGG 10 8 17 8 24 0 100 5 10
    240606225912_7l0y TCAGTGTCAATGAGCAGTGGTCATATGCTTTG(+ATATAT)GATCCAAGAACAGTTAAAAGGGAAAAATACA NGG 10 8 17 8 24 0 100 5 10
    240606225911_iiyw ATGGGAAAGTCAGGGGAGAACTGGGGCTAGAG(+ATATAT)GTACCCTGTAAACCAGTTTCTAGATAAAAAC NGG 10 8 17 8 24 0 100 5 10
    240606225909_ix6z GAGAAGTTTTTATCTAGAAACTGGTTTACAGG(+ATATAT)GTACCTCTAGCCCCAGTTCTCCCCTGACTTT NGG 10 8 17 8 24 0 100 5 10
    240606225907_nbl5 AAAGTAAGCAAATTGTAGCATAAGATATATTG(+ATATAT)GATCCACTCAGGAAGGTTGTATTACATTAGG NGG 10 8 17 8 24 0 100 5 10
    240606225906_4i97 TATTTCCTAATGTAATACAACCTTCCTGAGTG(+ATATAT)GATCCAATATATCTTATGCTACAATTTGCTT NGG 10 8 17 8 24 0 100 5 10
    240606225903_zjw6 GGAACAAAGCACCATTGGCCTCTAGCTCTGGG(+ATATAT)GATCCTACAGCGTCACCTTCCTCCTAGACAG NGG 10 8 17 8 24 0 100 5 10