Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606223252_ahpd CACAGGCAAGACCTCCTCCAACTCTACACCTG(+ATATAT)GTACCCAGGGCCTGCAGCAGTGGCAGGATCT NGG 10 8 17 8 24 0 100 5 10
    240606223249_umlm AGCTCAGATCCTGCCACTGCTGCAGGCCCTGG(+ATATAT)GTACCAGGTGTAGAGTTGGAGGAGGTCTTGC NGG 10 8 17 8 24 0 100 5 10
    240606223247_spdr TCACTCAGAAATATATTTTCTTTTCTTTCTGG(+ATATAT)GTACCCTTTAGAAATGGTACTAGTCATTCCC NGG 10 8 17 8 24 0 100 5 10
    240606223245_1yvo AGAAAGGGAATGACTAGTACCATTTCTAAAGG(+ATATAT)GTACCCAGAAAGAAAAGAAAATATATTTCTG NGG 10 8 17 8 24 0 100 5 10
    240606223243_8896 TAAATGTTGTGTTGTTTCTTTCTTCTCAGAGG(+ATATAT)GATCCTGTAATATCACCAAGGCCTTCTTTAT NGG 10 8 17 8 24 0 100 5 10
    240606223242_p9ii CTGGTATAAAGAAGGCCTTGGTGATATTACAG(+ATATAT)GATCCCTCTGAGAAGAAAGAAACAACACAAC NGG 10 8 17 8 24 0 100 5 10
    240606223240_hskm GCAAATATTTGCTCTTTTCCCAGATTGTCCTG(+ATATAT)GTACCTACCCCTTGTACACATCCCTGCCTAT NGG 10 8 17 8 24 0 100 5 10
    240606223238_8rrv CTTTTATAGGCAGGGATGTGTACAAGGGGTAG(+ATATAT)GTACCAGGACAATCTGGGAAAAGAGCAAATA NGG 10 8 17 8 24 0 100 5 10
    240606223236_xyrt TTCTTCATGAACGATATTTGACATGTGCTTTG(+ATATAT)GTACCCTTCTCTGAAAGTTGAAAACCTACCT NGG 10 8 17 8 24 0 100 5 10
    240606223234_7bpu TAAGTAGGTAGGTTTTCAACTTTCAGAGAAGG(+ATATAT)GTACCAAAGCACATGTCAAATATCGTTCATG NGG 10 8 17 8 24 0 100 5 10