Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606223155_ehcy TTCTGGGTATGTGTCCAGGAGAATAATTGCTG(+ATATAT)GATCCTCCTGTAGTACTATGAACTATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240606223154_xovb TACTCAGAAAATAGTTCATAGTACTACAGGAG(+ATATAT)GATCCAGCAATTATTCTCCTGGACACATACC NGG 10 8 17 8 24 0 100 5 10
    240606223152_lrln CACAATTTTCCTTTTCATCCCCCACCACATTG(+ATATAT)GATCCTAAATATATACCATCACTTTTCTCAT NGG 10 8 17 8 24 0 100 5 10
    240606223151_za3p CCTCTATGAGAAAAGTGATGGTATATATTTAG(+ATATAT)GATCCAATGTGGTGGGGGATGAAAAGGAAAA NGG 10 8 17 8 24 0 100 5 10
    240606223149_3sqh GTAAAAGGTCTACAATACCAGATGCGAGTCGG(+ATATAT)GATCCAAATCACCCAGCCTAACTGTGCTTAG NGG 10 8 17 8 24 0 100 5 10
    240606223148_lwuw TGTAGCTAAGCACAGTTAGGCTGGGTGATTTG(+ATATAT)GATCCCGACTCGCATCTGGTATTGTAGACCT NGG 10 8 17 8 24 0 100 5 10
    240606223146_k3rl ATGGAGAGGGTGTGCACTAACTGAAACTTTTG(+ATATAT)GATCCAAGGACAAGACAATCTTTTCCTTATC NGG 10 8 17 8 24 0 100 5 10
    240606223145_3ime AAACTGATAAGGAAAAGATTGTCTTGTCCTTG(+ATATAT)GATCCAAAAGTTTCAGTTAGTGCACACCCTC NGG 10 8 17 8 24 0 100 5 10
    240606223142_dgtm GTGCTAAAGAGATTTTCACAGACATCTTCCAG(+ATATAT)GTACCTGGTCTAGTTAGTGGCAGGGATATGT NGG 10 8 17 8 24 0 100 5 10
    240606223141_xvg5 GTAAAACATATCCCTGCCACTAACTAGACCAG(+ATATAT)GTACCTGGAAGATGTCTGTGAAAATCTCTTT NGG 10 8 17 8 24 0 100 5 10