Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606223232_mb6o AGAAAGGGCTGCAGTCAGTTTGACTAAAACGG(+ATATAT)GATCCACGTTATTGCTTAGAAAGGAAGCAAG NGG 10 8 17 8 24 0 100 5 10
    240606223230_mft4 GCTGGCTTGCTTCCTTTCTAAGCAATAACGTG(+ATATAT)GATCCCGTTTTAGTCAAACTGACTGCAGCCC NGG 10 8 17 8 24 0 100 5 10
    240606223228_hohs AGTATAGTATCAATAACGTCTACATCACCAGG(+ATATAT)GTACCTTTGCATTAAATGAGTTTTGCAAAAT NGG 10 8 17 8 24 0 100 5 10
    240606223227_3m92 TTACTATTTTGCAAAACTCATTTAATGCAAAG(+ATATAT)GTACCCTGGTGATGTAGACGTTATTGATACT NGG 10 8 17 8 24 0 100 5 10
    240606223225_d9wz CAAAGATTAAAGAGGACCAAAAGATGAGTTCG(+ATATAT)GTACCATAACAAAAAAAATCTCACCTACTGT NGG 10 8 17 8 24 0 100 5 10
    240606223224_qi0y ATGGTACAGTAGGTGAGATTTTTTTTGTTATG(+ATATAT)GTACCGAACTCATCTTTTGGTCCTCTTTAAT NGG 10 8 17 8 24 0 100 5 10
    240606223223_3qkx ACTGTCTGGGTTTGGTGACTGATAATGGAATG(+ATATAT)GATCCCCAGTTAGAGCAGTCTCTGGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240606223219_jzty GGAATGACCATCCAGAGACTGCTCTAACTGGG(+ATATAT)GATCCATTCCATTATCAGTCACCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240606223217_zze6 TTCTGGGTATGTGTCCAGGAGCATAATTGCTG(+ATATAT)GATCCTCCTGTAGTACTGTGAACTATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240606223215_vkv0 TACTCAGAAAATAGTTCACAGTACTACAGGAG(+ATATAT)GATCCAGCAATTATGCTCCTGGACACATACC NGG 10 8 17 8 24 0 100 5 10