Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606223119_s3s8 GCACTGCAGAAAGCATCTGAAAATCGTCCTTG(+ATATAT)GATCCCATGTGAGTGGTGGCACCACTAAGTC NGG 10 8 17 8 24 0 100 5 10
    240606223117_oxy1 GCAGAGACTTAGTGGTGCCACCACTCACATGG(+ATATAT)GATCCAAGGACGATTTTCAGATGCTTTCTGC NGG 10 8 17 8 24 0 100 5 10
    240606223116_d3q3 GTCAAGTTCACCATGTGCTTAAATAACAGATG(+ATATAT)GATCCTAGGCTAGAGGGTTTTCTCCTCAGCA NGG 10 8 17 8 24 0 100 5 10
    240606223113_33s5 AGCTTTGCTGAGGAGAAAACCCTCTAGCCTAG(+ATATAT)GATCCATCTGTTATTTAAGCACATGGTGAAC NGG 10 8 17 8 24 0 100 5 10
    240606223112_3af2 AAACACCTGTTAGTTCTAACTGTGTAGATTGG(+ATATAT)GTACCATAGTTAAGCTTAAGGATCCGGGTGT NGG 10 8 17 8 24 0 100 5 10
    240606223110_n0ax CGTTCACACCCGGATCCTTAAGCTTAACTATG(+ATATAT)GTACCCAATCTACACAGTTAGAACTAACAGG NGG 10 8 17 8 24 0 100 5 10
    240606223109_xx5j TGTGTAGATTGGGTACCATAGTTAAGCTTAAG(+ATATAT)GATCCGGGTGTGAACGGCACACAAGCTGGGG NGG 10 8 17 8 24 0 100 5 10
    240606223106_172x AGAAGCCCCAGCTTGTGTGCCGTTCACACCCG(+ATATAT)GATCCTTAAGCTTAACTATGGTACCCAATCT NGG 10 8 17 8 24 0 100 5 10
    240606223105_i722 GCGAGGGGACACAGCAACCGTGCAAACGTCAG(+ATATAT)GATCCTGCATTCTTTAGATTGTTTCCTTTTA NGG 10 8 17 8 24 0 100 5 10
    240606223104_a8a9 TAATTTAAAAGGAAACAATCTAAAGAATGCAG(+ATATAT)GATCCTGACGTTTGCACGGTTGCTGTGTCCC NGG 10 8 17 8 24 0 100 5 10