Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606223046_5glj ACCTTTACAGACTTGCCCCTTGCCTCACACAG(+ATATAT)GATCCAATCAAATTTAATTCAAGTCTCAATG NGG 10 8 17 8 24 0 100 5 10
    240606223044_9eg5 TTTCACATTGAGACTTGAATTAAATTTGATTG(+ATATAT)GATCCTGTGTGAGGCAAGGGGCAAGTCTGTA NGG 10 8 17 8 24 0 100 5 10
    240606223042_032y AATGAGTCAGAATTGGCCCTACAGCCCCTTTG(+ATATAT)GATCCTGCAGGATACCCTAGTGGCTAAACCT NGG 10 8 17 8 24 0 100 5 10
    240606223041_mqyg GGGCTAGGTTTAGCCACTAGGGTATCCTGCAG(+ATATAT)GATCCAAAGGGGCTGTAGGGCCAATTCTGAC NGG 10 8 17 8 24 0 100 5 10
    240606223039_7qx4 TGGGGCAGCTGTCAAAAGCTGCAGTTGGATAG(+ATATAT)GTACCAGCCTGTGGTGACTGGTAAGTAGTGG NGG 10 8 17 8 24 0 100 5 10
    240606223038_7zh7 GTTTGCCACTACTTACCAGTCACCACAGGCTG(+ATATAT)GTACCTATCCAACTGCAGCTTTTGACAGCTG NGG 10 8 17 8 24 0 100 5 10
    240606223036_bsww GTCCCAGTTTCCACCTTCCTCTGGCCCAGTAG(+ATATAT)GATCCCCAATCTGACTCTTTCAAGGCTCAGA NGG 10 8 17 8 24 0 100 5 10
    240606223034_21h0 GAATGTCTGAGCCTTGAAAGAGTCAGATTGGG(+ATATAT)GATCCTACTGGGCCAGAGGAAGGTGGAAACT NGG 10 8 17 8 24 0 100 5 10
    240606223032_h9hu GGTAACCACATTTTTCAACTCCACCCACCATG(+ATATAT)GTACCCTAGAACCAAGACTGGAATACTGACT NGG 10 8 17 8 24 0 100 5 10
    240606223031_p0zz TCTCCAGTCAGTATTCCAGTCTTGGTTCTAGG(+ATATAT)GTACCATGGTGGGTGGAGTTGAAAAATGTGG NGG 10 8 17 8 24 0 100 5 10