Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606223029_ctgk ACCACATGGTGGCCCACAACCATCTGTAATGG(+ATATAT)GATCCAATGCCCTCTTTTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240606223028_ar4k GCTGTCTTCAGACACACCAAAAGAGGGCATTG(+ATATAT)GATCCCATTACAGATGGTTGTGGGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240606223026_0g9j TTAGAGTGGTGGGTTGGTCAGCTGGAAGACAG(+ATATAT)GATCCAAAATGTTCCATGGAAGGCTGTCTTT NGG 10 8 17 8 24 0 100 5 10
    240606223025_fmb1 AGCCTAAAGACAGCCTTCCATGGAACATTTTG(+ATATAT)GATCCTGTCTTCCAGCTGACCAACCCACCAC NGG 10 8 17 8 24 0 100 5 10
    240606223024_lu1v GCTGAGGGGCTGAGTTCCCTTGAAGCAGCCAG(+ATATAT)GTACCGGTGGCCAGCTCTCTCTTGTTCTAGA NGG 10 8 17 8 24 0 100 5 10
    240606223021_nza2 TGGACTCTAGAACAAGAGAGAGCTGGCCACCG(+ATATAT)GTACCTGGCTGCTTCAAGGGAACTCAGCCCC NGG 10 8 17 8 24 0 100 5 10
    240606223019_wptf CAGCATCACCTGCCCCTACCCAGTGCCCACAG(+ATATAT)GTACCAGTAAACCTTAGGAACACAAAGTCAG NGG 10 8 17 8 24 0 100 5 10
    240606223018_h49j TGAAGCTGACTTTGTGTTCCTAAGGTTTACTG(+ATATAT)GTACCTGTGGGCACTGGGTAGGGGCAGGTGA NGG 10 8 17 8 24 0 100 5 10
    240606223016_d2as ATTTTCTCTTTATAACCTTGGTTTTGTTGGAG(+ATATAT)GATCCTCACCTAGAAAGTTGAAGACAGAGAT NGG 10 8 17 8 24 0 100 5 10
    240606223014_7m39 CATTTATCTCTGTCTTCAACTTTCTAGGTGAG(+ATATAT)GATCCTCCAACAAAACCAAGGTTATAAAGAG NGG 10 8 17 8 24 0 100 5 10