Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606225415_7br3 AAACAGAAGCGCTGGAATGATTGCCTCGCCAG(+ATATAT)GTACCGAGAAGAGCACTTTTAGGGACTGGTT NGG 10 8 17 8 24 0 100 5 10
    240606225413_n7uo AGAATCTAGTAGCCACACAATGGGGGGCTCAG(+ATATAT)GTACCATTATTTACTACTTTCTTTTGACCTA NGG 10 8 17 8 24 0 100 5 10
    240606225412_lmwv AGTCCTAGGTCAAAAGAAAGTAGTAAATAATG(+ATATAT)GTACCTGAGCCCCCCATTGTGTGGCTACTAG NGG 10 8 17 8 24 0 100 5 10
    240606225408_lsd4 AAATACTCGATGTGCAATTACTTTGCACTTGG(+ATATAT)GATCCAGCAAGCCCCTCAGCGGTATCCTGAT NGG 10 8 17 8 24 0 100 5 10
    240606225407_7ayn TGGTTATCAGGATACCGCTGAGGGGCTTGCTG(+ATATAT)GATCCCAAGTGCAAAGTAATTGCACATCGAG NGG 10 8 17 8 24 0 100 5 10
    240606225405_bwfx AATGACATGAACTTATTAAGAATTAACTTAAG(+ATATAT)GATCCAATATATATATATATATATAATATGT NGG 10 8 17 8 24 0 100 5 10
    240606225403_2b41 ATATAACATATTATATATATATATATATATTG(+ATATAT)GATCCTTAAGTTAATTCTTAATAAGTTCATG NGG 10 8 17 8 24 0 100 5 10
    240606225401_htbj TGCTTAATTCTTTTAGCGAATGCACAGGTTAG(+ATATAT)GTACCACCGTGAGGAAAAGGAGGGTGTGGGG NGG 10 8 17 8 24 0 100 5 10
    240606225359_itql TCATTCCCCACACCCTCCTTTTCCTCACGGTG(+ATATAT)GTACCTAACCTGTGCATTCGCTAAAAGAATT NGG 10 8 17 8 24 0 100 5 10
    240606225356_1jl9 TGCCTTCATCTCAGAATTGCTCATCCCAGGTG(+ATATAT)GATCCCATGTGTCTGCAGTAGACCAGTCACC NGG 10 8 17 8 24 0 100 5 10