Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606222952_quom GTTACACCTAGGCTGCAGGAAGGATGCTGGAG(+ATATAT)GTACCCTGGCCTCACCTGGTTTAGGACAGGA NGG 10 8 17 8 24 0 100 5 10
    240606222950_bh3t CACATTCCTGTCCTAAACCAGGTGAGGCCAGG(+ATATAT)GTACCTCCAGCATCCTTCCTGCAGCCTAGGT NGG 10 8 17 8 24 0 100 5 10
    240606222948_79cp ACGGCCCCAAGAAATGCTGAGATTTTACAATG(+ATATAT)GATCCATACTCCATTAAGAGATGTGTGAGTT NGG 10 8 17 8 24 0 100 5 10
    240606222947_y8xy GTATGAACTCACACATCTCTTAATGGAGTATG(+ATATAT)GATCCATTGTAAAATCTCAGCATTTCTTGGG NGG 10 8 17 8 24 0 100 5 10
    240606222945_6uxx ACCCCCTGCAGTGGGCTAATTAGGACTGAGGG(+ATATAT)GTACCTCTAAGGCTTTGGGGGTTCAACAGAG NGG 10 8 17 8 24 0 100 5 10
    240606222944_k5pw GAGGCCTCTGTTGAACCCCCAAAGCCTTAGAG(+ATATAT)GTACCCCTCAGTCCTAATTAGCCCACTGCAG NGG 10 8 17 8 24 0 100 5 10
    240606222941_mn7b AGAAGGGGACCCTGGCCACAGGACACTTCCAG(+ATATAT)GATCCATGCTCCCCTCCTCCTTGGGTCTGAG NGG 10 8 17 8 24 0 100 5 10
    240606222939_qm0f TCAAACTCAGACCCAAGGAGGAGGGGAGCATG(+ATATAT)GATCCTGGAAGTGTCCTGTGGCCAGGGTCCC NGG 10 8 17 8 24 0 100 5 10
    240606222936_fxbv GCTCCTGAGACTTCCCACTTTTAGCAGTTGGG(+ATATAT)GTACCAGGCCAGCAGCACCAGGGGCCAGATG NGG 10 8 17 8 24 0 100 5 10
    240606222935_nkv0 CAACCCATCTGGCCCCTGGTGCTGCTGGCCTG(+ATATAT)GTACCCCAACTGCTAAAAGTGGGAAGTCTCA NGG 10 8 17 8 24 0 100 5 10