Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606223214_q33p CACAATTTTCCTTTTCATCCCCCACCACATTG(+ATATAT)GATCCTAAATATATACCATCACTTTTCTCGT NGG 10 8 17 8 24 0 100 5 10
    240606223213_5i9m CCTCTACGAGAAAAGTGATGGTATATATTTAG(+ATATAT)GATCCAATGTGGTGGGGGATGAAAAGGAAAA NGG 10 8 17 8 24 0 100 5 10
    240606223210_v89r ACTGTCTGGGTTTGGTGACTGATAATGGAATG(+ATATAT)GATCCCCAGGTAGAGCAGTCTCTGGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240606223208_20wl GGAATGACCATCCAGAGACTGCTCTACCTGGG(+ATATAT)GATCCATTCCATTATCAGTCACCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240606223205_yloe TTCTGGGTATGTGTCCAGGAGAATAATTGCTG(+ATATAT)GATCCTCCTGTAGTACTGTGAACTATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240606223204_gjl8 TACTCAGAAAATAGTTCACAGTACTACAGGAG(+ATATAT)GATCCAGCAATTATTCTCCTGGACACATACC NGG 10 8 17 8 24 0 100 5 10
    240606223202_63qq CACAATTTTCCTTTTCATCCCCCACCACATTG(+ATATAT)GATCCTAAATATATACCATCACTTTTCTCGT NGG 10 8 17 8 24 0 100 5 10
    240606223201_kogn CCTCTACGAGAAAAGTGATGGTATATATTTAG(+ATATAT)GATCCAATGTGGTGGGGGATGAAAAGGAAAA NGG 10 8 17 8 24 0 100 5 10
    240606223200_9pv8 ACTGTCTGGGTTTGGTGACTGATGATGGAATG(+ATATAT)GATCCCCAGGTAGAGCAGTCTCTGGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240606223157_crky GGAATGACCATCCAGAGACTGCTCTACCTGGG(+ATATAT)GATCCATTCCATCATCAGTCACCAAACCCAG NGG 10 8 17 8 24 0 100 5 10