Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606223327_y2kl TTCTGCCACTCCCTCATGCATGTTTGAGGGTG(+ATATAT)GATCCCATCCAATGACCAGTAAGAGATTAAA NGG 10 8 17 8 24 0 100 5 10
    240606223325_u9iv AAGCTTTTAATCTCTTACTGGTCATTGGATGG(+ATATAT)GATCCACCCTCAAACATGCATGAGGGAGTGG NGG 10 8 17 8 24 0 100 5 10
    240606223323_56lf CCACCCCAGCCAAGAGCTGAGGCGCATGAGAG(+ATATAT)GATCCTGAGCATCCCCAGAAGGGTGTGTCTG NGG 10 8 17 8 24 0 100 5 10
    240606223322_o4jm CACAGCAGACACACCCTTCTGGGGATGCTCAG(+ATATAT)GATCCTCTCATGCGCCTCAGCTCTTGGCTGG NGG 10 8 17 8 24 0 100 5 10
    240606223320_4y7h CAGGTGGCTAACAACTGCCTTTAACTTCAAGG(+ATATAT)GATCCAAAGCCCTCCTCAGGCACCTGTACCC NGG 10 8 17 8 24 0 100 5 10
    240606223318_1l5u TATGTGGGTACAGGTGCCTGAGGAGGGCTTTG(+ATATAT)GATCCCTTGAAGTTAAAGGCAGTTGTTAGCC NGG 10 8 17 8 24 0 100 5 10
    240606223317_gp0h AAGTTCTTACAGTTTCCAGACTATAATTTTAG(+ATATAT)GATCCAGAAAGGACTCGGAGAACGCATCCCC NGG 10 8 17 8 24 0 100 5 10
    240606223315_zsvt TTGTGGGGGATGCGTTCTCCGAGTCCTTTCTG(+ATATAT)GATCCTAAAATTATAGTCTGGAAACTGTAAG NGG 10 8 17 8 24 0 100 5 10
    240606223313_db2v AGCCTGTGAGTTATATCCCCCCAGCAGCCATG(+ATATAT)GATCCCATGGCTTCAACAGCGGGTGTACTTG NGG 10 8 17 8 24 0 100 5 10
    240606223311_oesy ATACACAAGTACACCCGCTGTTGAAGCCATGG(+ATATAT)GATCCATGGCTGCTGGGGGGATATAACTCAC NGG 10 8 17 8 24 0 100 5 10