Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606223345_hc7v GTTCTCAAAACAAATGTTCTTAGTTCTCAGTG(+ATATAT)GATCCTGCAGATCTTAAATCATTCTTTGCGT NGG 10 8 17 8 24 0 100 5 10
    240606223343_ln65 TAAAAACGCAAAGAATGATTTAAGATCTGCAG(+ATATAT)GATCCACTGAGAACTAAGAACATTTGTTTTG NGG 10 8 17 8 24 0 100 5 10
    240606223341_ii6x GCAGTCTTTTTAGCAGTAGACTCAGAAGTCAG(+ATATAT)GATCCTCAGACCAACTCAATATATACTTTTA NGG 10 8 17 8 24 0 100 5 10
    240606223340_tzq1 AAAACTAAAAGTATATATTGAGTTGGTCTGAG(+ATATAT)GATCCTGACTTCTGAGTCTACTGCTAAAAAG NGG 10 8 17 8 24 0 100 5 10
    240606223338_iokw TTGGCTGGTACTTCCTTCCTTCCAGGAGATGG(+ATATAT)GATCCCTCTAGATACAGGGCCTAGAGACCAA NGG 10 8 17 8 24 0 100 5 10
    240606223336_mtfm AGGTTTTGGTCTCTAGGCCCTGTATCTAGAGG(+ATATAT)GATCCCATCTCCTGGAAGGAAGGAAGTACCA NGG 10 8 17 8 24 0 100 5 10
    240606223335_e2ub ATTTTGCTTCATGTATCCAGGTCCTCTGCAGG(+ATATAT)GTACCAAGGAATGTTCTTCAGGATAGCGGAA NGG 10 8 17 8 24 0 100 5 10
    240606223333_s3jc AATTGTTCCGCTATCCTGAAGAACATTCCTTG(+ATATAT)GTACCCTGCAGAGGACCTGGATACATGAAGC NGG 10 8 17 8 24 0 100 5 10
    240606223332_75rh GTCAAAGTATGTTTAACATATGATTTCTTCAG(+ATATAT)GATCCCTCCCCTTTCTGAATTCCAAATGGAG NGG 10 8 17 8 24 0 100 5 10
    240606223329_tft9 AGTTCCTCCATTTGGAATTCAGAAAGGGGAGG(+ATATAT)GATCCTGAAGAAATCATATGTTAAACATACT NGG 10 8 17 8 24 0 100 5 10