Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606225902_rq8k TCCTGCTGTCTAGGAGGAAGGTGACGCTGTAG(+ATATAT)GATCCCCAGAGCTAGAGGCCAATGGTGCTTT NGG 10 8 17 8 24 0 100 5 10
    240606225900_fbor AAGGTTTGCCCTTTAGTTTAGAAACCAGATAG(+ATATAT)GATCCCATCCTTGAATTTTAAATGAAGTCAT NGG 10 8 17 8 24 0 100 5 10
    240606225857_fa6d TGCAAATGACTTCATTTAAAATTCAAGGATGG(+ATATAT)GATCCTATCTGGTTTCTAAACTAAAGGGCAA NGG 10 8 17 8 24 0 100 5 10
    240606225856_udkp GCAGTGCTGGTAGAATCCAGTAGAGGATATTG(+ATATAT)GATCCTCTGTGACTAGAATTACAGATAGTTA NGG 10 8 17 8 24 0 100 5 10
    240606225855_22qx GTTCATAACTATCTGTAATTCTAGTCACAGAG(+ATATAT)GATCCAATATCCTCTACTGGATTCTACCAGC NGG 10 8 17 8 24 0 100 5 10
    240606225853_382m CCAGACAAATGTAGTTTACTGCAGGCTTACTG(+ATATAT)GATCCTGACATTTAATTAAATATAACATAAT NGG 10 8 17 8 24 0 100 5 10
    240606225851_sxtj ACTAGATTATGTTATATTTAATTAAATGTCAG(+ATATAT)GATCCAGTAAGCCTGCAGTAAACTACATTTG NGG 10 8 17 8 24 0 100 5 10
    240606225849_nu1h ATAACATGGCAATAATGAAGTGCTGTTTCCTG(+ATATAT)GTACCTTGGGAATACTAAAGAAAGAAAAAGC NGG 10 8 17 8 24 0 100 5 10
    240606225846_kq16 GCAATGCTTTTTCTTTCTTTAGTATTCCCAAG(+ATATAT)GTACCAGGAAACAGCACTTCATTATTGCCAT NGG 10 8 17 8 24 0 100 5 10
    240606225844_7aik TTAAAAGAGAAACCAGAAACATCTAATCTGAG(+ATATAT)GATCCAGACAATAAATATTACATGGATCATT NGG 10 8 17 8 24 0 100 5 10