Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606223643_oi2s TGGAGTGAACAATACTGAGTGGCATATGCTTG(+ATATAT)GATCCGTCCTCTCTTCTAAGGGTTGTGAGCC NGG 10 8 17 8 24 0 100 5 10
    240606223642_dt2n AAGTAGGCTCACAACCCTTAGAAGAGAGGACG(+ATATAT)GATCCAAGCATATGCCACTCAGTATTGTTCA NGG 10 8 17 8 24 0 100 5 10
    240606223640_a1a6 TACCACCCTGGGAAAAGTCACATTCGAGGCTG(+ATATAT)GATCCTACTGAAGTCAAGGCTTGTTCGTTTA NGG 10 8 17 8 24 0 100 5 10
    240606223638_dutg GCTGTTAAACGAACAAGCCTTGACTTCAGTAG(+ATATAT)GATCCAGCCTCGAATGTGACTTTTCCCAGGG NGG 10 8 17 8 24 0 100 5 10
    240606223637_be4n TTGCGTGCCACCCGCAGCCCCCAACCTTGTAG(+ATATAT)GATCCGGGCGATCCCCATGAGGCTCCCTGAA NGG 10 8 17 8 24 0 100 5 10
    240606223634_cqay CTGAGTTCAGGGAGCCTCATGGGGATCGCCCG(+ATATAT)GATCCTACAAGGTTGGGGGCTGCGGGTGGCA NGG 10 8 17 8 24 0 100 5 10
    240606223632_q929 CGCCCTCTAGTGACCGGGAGGCCAGTAACAGG(+ATATAT)GATCCCTTTCTCCAAATGACCAAATGTTGGG NGG 10 8 17 8 24 0 100 5 10
    240606223630_rh8o ATCGACCCAACATTTGGTCATTTGGAGAAAGG(+ATATAT)GATCCCTGTTACTGGCCTCCCGGTCACTAGA NGG 10 8 17 8 24 0 100 5 10
    240606223629_zeo4 ATTTATACATAAAACTCCCAAGGAACCATGTG(+ATATAT)GATCCGACAGAATGTTAATAGCACATCTAAG NGG 10 8 17 8 24 0 100 5 10
    240606223628_5wnu TTATTCTTAGATGTGCTATTAACATTCTGTCG(+ATATAT)GATCCACATGGTTCCTTGGGAGTTTTATGTA NGG 10 8 17 8 24 0 100 5 10