Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606230224_hfv6 TCCAGGGGATACACTACCTCTGGGCTCCATGG(+ATATAT)GTACCTCTCCACACAAGTGGTGTACACTCAC NGG 10 8 17 8 24 0 100 5 10
    240606230222_9gha TGCACACACCCATCTTCGCTACTCCAGACTAG(+ATATAT)GATCCATAGCAATTGGGAAATGGGCATTGGG NGG 10 8 17 8 24 0 100 5 10
    240606230221_xo7w TACTCCCCAATGCCCATTTCCCAATTGCTATG(+ATATAT)GATCCTAGTCTGGAGTAGCGAAGATGGGTGT NGG 10 8 17 8 24 0 100 5 10
    240606230219_8bry CCCAGGAAATGCCTCCCTCCGGTCCAGGCAGG(+ATATAT)GATCCCAGCACACACACCCCTGCTGGTGGTG NGG 10 8 17 8 24 0 100 5 10
    240606230217_zyoy CCCTCCACCACCAGCAGGGGTGTGTGTGCTGG(+ATATAT)GATCCCTGCCTGGACCGGAGGGAGGCATTTC NGG 10 8 17 8 24 0 100 5 10
    240606230214_rkw5 GTCCCACTTTCAATGGGAGCTATGAGGTATGG(+ATATAT)GTACCAGGCTTAGCAAGCAAGTTGTGGTCCC NGG 10 8 17 8 24 0 100 5 10
    240606230212_3zpc CTGTGGGGACCACAACTTGCTTGCTAAGCCTG(+ATATAT)GTACCCATACCTCATAGCTCCCATTGAAAGT NGG 10 8 17 8 24 0 100 5 10
    240606230210_flqr TTGTTCAGGGACTACTCAAGAAAATATCTTTG(+ATATAT)GATCCTTAGTTGGCCTTCAAGTCTATACATA NGG 10 8 17 8 24 0 100 5 10
    240606230209_1f1j TCTTATATGTATAGACTTGAAGGCCAACTAAG(+ATATAT)GATCCAAAGATATTTTCTTGAGTAGTCCCTG NGG 10 8 17 8 24 0 100 5 10
    240606230205_8r85 ACACAGTTTGAAGCACTATGTTTCTTCCCCTG(+ATATAT)GTACCCTCTCTGTTCACAACTGCAGAAGCCT NGG 10 8 17 8 24 0 100 5 10