Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606223731_3dra AATCTTCTCTACCCAAATCCTCCTCATAAATG(+ATATAT)GATCCATGATGACAGCTAAGAGAAAAAAAAT NGG 10 8 17 8 24 0 100 5 10
    240606223730_8jff TACTTATTTTTTTTCTCTTAGCTGTCATCATG(+ATATAT)GATCCATTTATGAGGAGGATTTGGGTAGAGA NGG 10 8 17 8 24 0 100 5 10
    240606223728_do8j TGCAAAGGACTGGAGATACAGGGTTAAGACAG(+ATATAT)GTACCATCTACATATATTCTGAACAATGCAG NGG 10 8 17 8 24 0 100 5 10
    240606223727_3jcd CTACACTGCATTGTTCAGAATATATGTAGATG(+ATATAT)GTACCTGTCTTAACCCTGTATCTCCAGTCCT NGG 10 8 17 8 24 0 100 5 10
    240606223725_rh5w GGGTAATGCAAAGAAAATGAGTTTTCCTTTGG(+ATATAT)GTACCAGTAATTGTCAAAGGAATGATTGCAG NGG 10 8 17 8 24 0 100 5 10
    240606223724_bjmw TGAATCTGCAATCATTCCTTTGACAATTACTG(+ATATAT)GTACCCAAAGGAAAACTCATTTTCTTTGCAT NGG 10 8 17 8 24 0 100 5 10
    240606223723_yksq GCTGCACTTGAGGATAAATACCGGAGCTGTAG(+ATATAT)GTACCACTGCACCTTGTGTACATGGAACCTA NGG 10 8 17 8 24 0 100 5 10
    240606223722_39tf ATGGATAGGTTCCATGTACACAAGGTGCAGTG(+ATATAT)GTACCTACAGCTCCGGTATTTATCCTCAAGT NGG 10 8 17 8 24 0 100 5 10
    240606223720_o6xh GAACTAGTCATAAAGAGTGAGGTTGGTGTGAG(+ATATAT)GTACCAGACATGTAAGAAACAAGGGGACACA NGG 10 8 17 8 24 0 100 5 10
    240606223719_d2zk GGTGGTGTGTCCCCTTGTTTCTTACATGTCTG(+ATATAT)GTACCTCACACCAACCTCACTCTTTATGACT NGG 10 8 17 8 24 0 100 5 10