Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606223824_615r CAAATGGTTTATTTTAAACTAGACCCATCATG(+ATATAT)GTACCTCAGGGAAAGATGTCTCAGCATGGAC NGG 10 8 17 8 24 0 100 5 10
    240606223821_r4na AGTGGGTCCATGCTGAGACATCTTTCCCTGAG(+ATATAT)GTACCATGATGGGTCTAGTTTAAAATAAACC NGG 10 8 17 8 24 0 100 5 10
    240606223819_jss6 TACCCAGGGAGATGCGAGGAGATAGATTCATG(+ATATAT)GTACCTGAGCACAGGTAACCAGCCACATGGT NGG 10 8 17 8 24 0 100 5 10
    240606223818_n3b4 ATTCTACCATGTGGCTGGTTACCTGTGCTCAG(+ATATAT)GTACCATGAATCTATCTCCTCGCATCTCCCT NGG 10 8 17 8 24 0 100 5 10
    240606223816_554k GGCTAACTGTAGCTGTATGTTAACAGTGGCAG(+ATATAT)GTACCCAGCTCCTCTCCCTGAGGCTACAGCT NGG 10 8 17 8 24 0 100 5 10
    240606223814_k3zg ATGGCAGCTGTAGCCTCAGGGAGAGGAGCTGG(+ATATAT)GTACCTGCCACTGTTAACATACAGCTACAGT NGG 10 8 17 8 24 0 100 5 10
    240606223813_v23y TAGTAGGAGTGGGAAATGGATTACTTTGTCTG(+ATATAT)GATCCCGAGTGGCTTTAGATTACACTCAGGG NGG 10 8 17 8 24 0 100 5 10
    240606223811_ench ATGTCCCCTGAGTGTAATCTAAAGCCACTCGG(+ATATAT)GATCCAGACAAAGTAATCCATTTCCCACTCC NGG 10 8 17 8 24 0 100 5 10
    240606223809_2ntm TGGCAGAGCCTCTCAGGAGACAGCTATATCGG(+ATATAT)GATCCTGTTAGCAAACACTTCTTGGCATCCA NGG 10 8 17 8 24 0 100 5 10
    240606223807_mdhv TATTGTGGATGCCAAGAAGTGTTTGCTAACAG(+ATATAT)GATCCCGATATAGCTGTCTCCTGAGAGGCTC NGG 10 8 17 8 24 0 100 5 10