Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606223932_ht95 AGAGCAAGAGGCTGGACTTGATGGTGCCGTTG(+ATATAT)GATCCCAGGTGCTCGCTCGCGGTTCTCATCC NGG 10 8 17 8 24 0 100 5 10
    240606223930_w9so CAAAAGGATGAGAACCGCGAGCGAGCACCTGG(+ATATAT)GATCCAACGGCACCATCAAGTCCAGCCTCTT NGG 10 8 17 8 24 0 100 5 10
    240606223929_577x GGGAAGCTCATCACATTGTAAGGCTCTCACTG(+ATATAT)GTACCACCCAGTCCTAATCAAGAATGGCATT NGG 10 8 17 8 24 0 100 5 10
    240606223927_0kp7 CCAGAAATGCCATTCTTGATTAGGACTGGGTG(+ATATAT)GTACCAGTGAGAGCCTTACAATGTGATGAGC NGG 10 8 17 8 24 0 100 5 10
    240606223926_y4dq CCTCAGGCCTGATCCATCATGCCCTGGACGCG(+ATATAT)GTACCCTAGAGCTTCTTAGCTGACTCTGGCT NGG 10 8 17 8 24 0 100 5 10
    240606223924_03ol GTGACAGCCAGAGTCAGCTAAGAAGCTCTAGG(+ATATAT)GTACCGCGTCCAGGGCATGATGGATCAGGCC NGG 10 8 17 8 24 0 100 5 10
    240606223922_f8s8 CTCAACGCAAACGAAGGGACTAGCTTATCCAG(+ATATAT)GATCCAACGTGCGACAGGGATAGCCTTAAAA NGG 10 8 17 8 24 0 100 5 10
    240606223921_jlkt AGTGCTTTTAAGGCTATCCCTGTCGCACGTTG(+ATATAT)GATCCTGGATAAGCTAGTCCCTTCGTTTGCG NGG 10 8 17 8 24 0 100 5 10
    240606223920_55jv GAATGCAAAAATAACTGCATTTTCCTGTTCAG(+ATATAT)GATCCAGTTGTATTTGACCATTCTAAATTAG NGG 10 8 17 8 24 0 100 5 10
    240606223919_rn10 TTTTACTAATTTAGAATGGTCAAATACAACTG(+ATATAT)GATCCTGAACAGGAAAATGCAGTTATTTTTG NGG 10 8 17 8 24 0 100 5 10