Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606224007_1gu0 TCTCAATGCAGAATTACTGTGTGCAGGGGGAG(+ATATAT)GTACCAGGTCCCTTTCATTTGGGCTCAACTC NGG 10 8 17 8 24 0 100 5 10
    240606224003_ld13 TGTTTGAGTTGAGCCCAAATGAAAGGGACCTG(+ATATAT)GTACCTCCCCCTGCACACAGTAATTCTGCAT NGG 10 8 17 8 24 0 100 5 10
    240606224001_1m5m ATATATGATGAAGGTGGGTCAGCTCTGGTTGG(+ATATAT)GATCCGAGAAGTGACACAGCTGGAGAATGTC NGG 10 8 17 8 24 0 100 5 10
    240606223959_b56b TAAGTGACATTCTCCAGCTGTGTCACTTCTCG(+ATATAT)GATCCCAACCAGAGCTGACCCACCTTCATCA NGG 10 8 17 8 24 0 100 5 10
    240606223958_f8xy AGTCAACAGGGACAGCAGAATGACCACTAATG(+ATATAT)GATCCTTAATCTTCTTCTAGTAGCTTTGGGG NGG 10 8 17 8 24 0 100 5 10
    240606223957_5tna ACTTCCCCCAAAGCTACTAGAAGAAGATTAAG(+ATATAT)GATCCATTAGTGGTCATTCTGCTGTCCCTGT NGG 10 8 17 8 24 0 100 5 10
    240606223955_oe56 GATGCAGAGATACGTCTGGGTGAGCTAACCAG(+ATATAT)GATCCTCATTACCTAGGTCATCAAGGGTCGA NGG 10 8 17 8 24 0 100 5 10
    240606223954_5n9q TACAATCGACCCTTGATGACCTAGGTAATGAG(+ATATAT)GATCCTGGTTAGCTCACCCAGACGTATCTCT NGG 10 8 17 8 24 0 100 5 10
    240606223952_1uia CTTCTTTTTTTTTTTTTTTTTTAGCAAAAGTG(+ATATAT)GTACCAGTGCAGGGCCAAGTGCGTTGCTCTC NGG 10 8 17 8 24 0 100 5 10
    240606223951_4wot CAGAAGAGAGCAACGCACTTGGCCCTGCACTG(+ATATAT)GTACCACTTTTGCTAAAAAAAAAAAAAAAAA NGG 10 8 17 8 24 0 100 5 10