Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606224659_2fwv AATACATTCAGACTTCAGCTCTCCAGAGGAAG(+ATATAT)GTACCCAGATCAATTTTTAGGTGGGAAAAAT NGG 10 8 17 8 24 0 100 5 10
    240606224657_200q CTGTCATTTTTCCCACCTAAAAATTGATCTGG(+ATATAT)GTACCTTCCTCTGGAGAGCTGAAGTCTGAAT NGG 10 8 17 8 24 0 100 5 10
    240606224655_v2z9 CAATTCGCTCCTTCTGTTCCCCACATTCCAGG(+ATATAT)GATCCTTCGGAGCAGGTTCTGTACGTCCAGC NGG 10 8 17 8 24 0 100 5 10
    240606224652_dy2i GTGGGGCTGGACGTACAGAACCTGCTCCGAAG(+ATATAT)GATCCCTGGAATGTGGGGAACAGAAGGAGCG NGG 10 8 17 8 24 0 100 5 10
    240606224649_y628 TTCTTCCACCCTTCTAGCCACTCTCTGGTTTG(+ATATAT)GTACCTCCTCAAAACATCTGGGCTCCTCTGC NGG 10 8 17 8 24 0 100 5 10
    240606224647_itc1 TGTATGCAGAGGAGCCCAGATGTTTTGAGGAG(+ATATAT)GTACCAAACCAGAGAGTGGCTAGAAGGGTGG NGG 10 8 17 8 24 0 100 5 10
    240606224644_37gf GGCTGTGTCCACACCAGCAGCAGGATCCTTGG(+ATATAT)GTACCCTTTGCTGCTTCCTTTCCCTTCCAGG NGG 10 8 17 8 24 0 100 5 10
    240606224642_vw18 TTGTGCCTGGAAGGGAAAGGAAGCAGCAAAGG(+ATATAT)GTACCCAAGGATCCTGCTGCTGGTGTGGACA NGG 10 8 17 8 24 0 100 5 10
    240606224640_9j4k GGGAAACTATTACTTCAAATGAACAGTATTGG(+ATATAT)GATCCTTCTGCCTTACTTAGTTACCTAGTAT NGG 10 8 17 8 24 0 100 5 10
    240606224638_62qj TTCAGATACTAGGTAACTAAGTAAGGCAGAAG(+ATATAT)GATCCCAATACTGTTCATTTGAAGTAATAGT NGG 10 8 17 8 24 0 100 5 10